Xing Bei-Bei, Huang Meng, Chen Guo-Hui, Zhang Di, Ding Guang-Hong
Department of Aeronautics and Astronautics, Fudan University, Shanghai 200433, China.
Department of Aeronautics and Astronautics, Fudan University, Shanghai 200433, China; Shanghai Research Center for Acupuncture and Meridians, Shanghai 201203.
Zhen Ci Yan Jiu. 2017 Aug 25;42(4):327-31.
To develop an animal model suitable for the study of acupoint sensitization in myocardial ischemia(MI) animals by observing changes of the mechanical withdrawal threshold (pain threshold, PT).
Twenty New Zea-land rabbits were randomly divided into control and model groups (=10 in each group). The controllable MI model was set up by installing a balloon occluder at the left anterior descending branch of the left coronary artery. Air inflation of the balloon was given for 5 min/time, twice a day (4-hours' interval) for continuous 5 days (the first stage of MI), with one day's rest, then, another 5 days' air inflation was conducted again (2 stage of MI). Electrocardiogram (ECG) of the standard limb lead Ⅱ was recorded for assessing MI, and myocardial 2,3,5-triphenyltetrazolium chloride (TTC) staining was used to verify the degree of ischemic myocardium. The PT of the skin areas of "Neiguan" (PC 6), "Shenmen" (HT 7),"Taixi"(KI 3),hind paw(HP)was detected under conscious conditions,before MI, on day 8(after recovery from MI), day 14 (the first stage of MI), and day 20 (the second stage of MI), respectively.
The controllable MI model was successful due to a marked elevation of ECG ST Ⅱ after inflation of the implanted balloon and a distinct recovery after balloon deflation (<0.05). The myocardial TTC staining showed that the area of the myocardial necrosis was about 10%. The PT values were significantly decreased in PC 6 area at the first stage of MI and in both PC 6 and HT 7 regions at the 2 stage of MI (<0.01), suggesting a close relationship between the acupoints of the Pericardium and Heart meridians and the heart in terms of the basic theory of Chinese medicine.
MI may induce a reduction of PT of some related acupoints in MI rabbits, and the controllable MI model of the present study is helpful to the study of acupoint sensitization phenomenon after occurrence of visceral diseases.
通过观察机械缩足阈值（疼痛阈值，PT）的变化，建立一种适用于研究心肌缺血（MI）动物穴位敏化的动物模型。
将20只新西兰兔随机分为对照组和模型组（每组10只）。通过在左冠状动脉前降支安装球囊阻塞器建立可控性MI模型。球囊充气5分钟/次，每天2次（间隔4小时），连续5天（MI第一阶段），休息1天，然后再次进行5天的球囊充气（MI第二阶段）。记录标准肢体导联Ⅱ的心电图（ECG）以评估MI，并使用心肌2,3,5-氯化三苯基四氮唑（TTC）染色来验证缺血心肌的程度。分别在MI前、MI后第8天（恢复后）、第14天（MI第一阶段）和第20天（MI第二阶段），在清醒状态下检测“内关”（PC 6）、“神门”（HT 7）、“太溪”（KI 3）、后爪（HP）皮肤区域的PT。
植入球囊充气后ECG STⅡ明显升高，球囊放气后明显恢复（P<0.05），可控性MI模型成功。心肌TTC染色显示心肌坏死面积约为10%。MI第一阶段PC 6区域的PT值显著降低，MI第二阶段PC 6和HT 7区域的PT值均显著降低（P<0.01），从中医基础理论来看，提示心包经和心经穴位与心脏之间存在密切关系。
MI可能导致MI兔某些相关穴位的PT降低，本研究的可控性MI模型有助于研究内脏疾病发生后穴位敏化现象。
