Kurta Ruslan P, Donatelli Jeffrey J, Yoon Chun Hong, Berntsen Peter, Bielecki Johan, Daurer Benedikt J, DeMirci Hasan, Fromme Petra, Hantke Max Felix, Maia Filipe R N C, Munke Anna, Nettelblad Carl, Pande Kanupriya, Reddy Hemanth K N, Sellberg Jonas A, Sierra Raymond G, Svenda Martin, van der Schot Gijs, Vartanyants Ivan A, Williams Garth J, Xavier P Lourdu, Aquila Andrew, Zwart Peter H, Mancuso Adrian P
European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany.
Mathematics Department, Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, California 94720, USA.
Phys Rev Lett. 2017 Oct 13;119(15):158102. doi: 10.1103/PhysRevLett.119.158102. Epub 2017 Oct 12.
We use extremely bright and ultrashort pulses from an x-ray free-electron laser (XFEL) to measure correlations in x rays scattered from individual bioparticles. This allows us to go beyond the traditional crystallography and single-particle imaging approaches for structure investigations. We employ angular correlations to recover the three-dimensional (3D) structure of nanoscale viruses from x-ray diffraction data measured at the Linac Coherent Light Source. Correlations provide us with a comprehensive structural fingerprint of a 3D virus, which we use both for model-based and ab initio structure recovery. The analyses reveal a clear indication that the structure of the viruses deviates from the expected perfect icosahedral symmetry. Our results anticipate exciting opportunities for XFEL studies of the structure and dynamics of nanoscale objects by means of angular correlations.
我们使用来自X射线自由电子激光（XFEL）的极亮且超短脉冲，来测量从单个生物粒子散射的X射线中的相关性。这使我们能够超越传统的晶体学和单粒子成像方法进行结构研究。我们利用角相关性，从在直线加速器相干光源处测得的X射线衍射数据中恢复纳米级病毒的三维（3D）结构。相关性为我们提供了3D病毒的全面结构指纹，我们将其用于基于模型和从头开始的结构恢复。分析结果清楚地表明，病毒的结构偏离了预期的完美二十面体对称性。我们的结果预示着通过角相关性对纳米级物体的结构和动力学进行XFEL研究将带来令人兴奋的机遇。
