Parin Ugur, Erbas Goksel, Kirkan Sukru, Savasan Serap, Tugba Yuksel H, Balat Gamze
Department of Microbiology, Veterinary Faculty, University of Adnan Menderes, 09016, Aydın, Turkey.
Trop Anim Health Prod. 2018 Feb;50(2):421-425. doi: 10.1007/s11250-017-1452-z. Epub 2017 Oct 28.
Systemic fungal diseases are the infections caused by false treatment protocols and generally are not taken into consideration especially in the veterinary field. One-humped camels are found in the western side of the Aegean region of our country and bred for wrestling. The aim of this study is the application of diagnosing systemic fungi infection from camel blood samples by the PCR method. In this study, specific primers for DNA topoisomerase II gene sequences were used. As a result, a systemic fungal infection was detected by the nested PCR method from 10 (20%) out of 50 DNA samples taken from camels located on the western side of the Aegean region. In this study, 3 (30%) samples were identified as Candida albicans, 3 (30%) samples were identified as C. glabrata, and 4 (40%) samples were identified as C. parapsilosis. In conclusion, the 20% positive systemic fungal infection rate in one-humped camels observed in the present study showed that the systemic fungal infections are not taken into considerations in veterinary medicine. Further studies are suggested in order to obtain and to maintain extensive data for systemic fungal diseases in our country for one-humped camels.
全身性真菌病是由错误的治疗方案引起的感染，通常未被考虑在内，尤其是在兽医领域。单峰骆驼分布在我国爱琴海地区的西部，用于摔跤比赛养殖。本研究的目的是应用聚合酶链反应（PCR）方法从骆驼血液样本中诊断全身性真菌感染。在本研究中，使用了针对DNA拓扑异构酶II基因序列的特异性引物。结果，通过巢式PCR方法从爱琴海地区西部的50份骆驼DNA样本中的10份（20%）检测到全身性真菌感染。在本研究中，3份（30%）样本被鉴定为白色念珠菌，3份（30%）样本被鉴定为光滑念珠菌，4份（40%）样本被鉴定为近平滑念珠菌。总之，本研究中观察到单峰骆驼全身性真菌感染阳性率为20%，表明兽医医学中未考虑全身性真菌感染。建议进一步开展研究，以便为我国单峰骆驼的全身性真菌病获取和保存广泛的数据。
