Xu Zhiru, Gao Liying, Tang Mengquan, Qu Chunpu, Huang Jiahuan, Wang Qi, Yang Chuanping, Liu Guanjun, Yang Chengjun
College of Life Science, Northeast Forestry University, HarBin, China.
State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry University, HarBin, China.
PeerJ. 2017 Oct 27;5:e3962. doi: 10.7717/peerj.3962. eCollection 2017.
Copper plays key roles in plant physiological activities. To maintain copper cellular homeostasis, copper chaperones have important functions in binding and transporting copper to target proteins. Detailed characterization and function analysis of a copper chaperone, CCH, is presently limited to . This study reports the identification of 21 genes encoding putative CCH proteins in . Besides sharing the conserved metal-binding motif MXCXXC and forming a βαββαβ secondary structure at the N-terminal, all the PtCCHs possessed the plant-exclusive extended C-terminal. Based on their gene structure, conserved motifs, and phylogenetic analysis, the PtCCHs were divided into three subgroups. Our analysis indicated that whole-genome duplication and tandem duplication events likely contributed to expansion of the gene family in . Tissue-specific data from PlantGenIE revealed that genes had broad expression patterns in different tissues. Quantitative real-time RT-PCR (qRT-PCR) analysis revealed that genes of × also had different tissue-specific expression traits, as well as different inducible-expression patterns in response to copper stresses (excessive and deficiency). In summary, our study of genes in the genome provides a comprehensive analysis of this gene family, and lays an important foundation for further investigation of their roles in copper homeostasis of poplar.
铜在植物生理活动中发挥着关键作用。为维持细胞内铜稳态，铜伴侣蛋白在将铜结合并转运至靶蛋白的过程中发挥着重要功能。目前，对一种铜伴侣蛋白CCH的详细表征和功能分析仅限于 。本研究报道了在 中鉴定出21个编码假定CCH蛋白的基因。除了共享保守的金属结合基序MXCXXC并在N端形成βαββαβ二级结构外，所有的PtCCH蛋白都具有植物特有的延长C端。基于它们的基因结构、保守基序和系统发育分析，PtCCH蛋白被分为三个亚组。我们的分析表明，全基因组复制和串联复制事件可能促成了 中 基因家族的扩张。来自PlantGenIE的组织特异性数据显示， 基因在不同组织中具有广泛的表达模式。定量实时RT-PCR（qRT-PCR）分析表明， × 的 基因也具有不同的组织特异性表达特征，以及在响应铜胁迫（过量和缺乏）时不同的诱导表达模式。总之，我们对 基因组中 基因的研究提供了对该基因家族的全面分析，并为进一步研究它们在杨树铜稳态中的作用奠定了重要基础。
