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对[具体物质1]和[具体物质2]甲醇提取物的气相色谱-质谱联用(GC-MS)分析及其抗氧化活性。

GC-MS analysis of the methanolic extracts of and and their antioxidant activity.

作者信息

Qadir Abdul, Aqil Mohd, Ali Athar, Ahmad Farhan J, Ahmad Sayeed, Arif Muhammad, Khan Nausheen

机构信息

Department of Pharmaceutics, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi, India.

Centre for Transgenic Plant Development, Department of Biotechnology, Jamia Hamdard, New Delhi India.

出版信息

Turk J Chem. 2020 Apr 1;44(2):352-363. doi: 10.3906/kim-1907-5. eCollection 2020.

Abstract

L. (family Smilacaceae) and L. (family Salicaceae) are plants that have been traditionally used to treat various ailments in Indian and Chinese medicine. A quantitative estimation of the methanolic extracts of these plants was performed by GC-MS analysis to obtain insight into its phytoconstituents responsible for therapeutic action. The antioxidant potential of the methanol extracts of (MESC) and (MESA) were assessed with DPPH by using a UV spectrophotometer at a wavelength of 517 nm. The prevailing compounds found in MESC were lactam sugars including 2,5-dimethyl-2,4-dihydroxy-3(2H)-furanon (1.40%), 1,5-anhydro-6-deoxyhexo-2,3-diulose (4.33%), and alpha-methyl-1-sorboside (1.80%); the two alkaloids found were 1,4-methane-4,4a,5,6,7,8,9,9a-octahydro-10,10-dimethyl cyclohepta[d] pyridazine (0.87%) and 1,3,7-trimethyl-2,6-dioxopurine(0.54%); terpenes included deltacadinene (0.39%), terpineol, (+)-cedrol (22.13%), 3-thujanol (0.77%), and 9,10-dehydro-cycloisolongifolene (0.34%); fatty acids included cis-vaccenic acid (4.98%) and telfairic acid (1.10%); esters included 1,2,3-propanetriol diacetate (7.56%), 7-hexadecenoic acid, methyl ester (1.77%), eicosanoic acid, and methyl ester (0.95%); and glycerol included 1,2,3-propanetriol (28.75%). The interesting compounds found in MESA were reducing sugars like D-allose (4.40%) and pyrogallol (10.48%), alkaloids like caffeine (63.49%), and esters like methyl octadecanoate (0.53%). Both fractions revealed considerable antioxidant activity. The reported existing phenolic compounds and terpenes are responsible for the antioxidant activity of the plant extracts.

摘要

菝葜属(菝葜科)植物和杨属(杨柳科)植物在印度和中国传统医学中一直被用于治疗各种疾病。通过气相色谱 - 质谱联用(GC - MS)分析对这些植物的甲醇提取物进行定量评估,以深入了解其具有治疗作用的植物成分。使用紫外可见分光光度计在波长517nm下,通过二苯基苦味酰基自由基(DPPH)法评估菝葜甲醇提取物(MESC)和杨属甲醇提取物(MESA)的抗氧化潜力。在MESC中发现的主要化合物包括内酰胺糖,如2,5 - 二甲基 - 2,4 - 二羟基 - 3(2H)-呋喃酮(1.40%)、1,5 - 脱水 - 6 - 脱氧己糖 - 2,3 - 二酮(4.33%)和α - 甲基 - 1 - 山梨糖苷(1.80%);发现的两种生物碱为1,4 - 亚甲基 - 4,4a,5,6,7,8,9,9a - 八氢 - 10,10 - 二甲基环庚并[d]哒嗪(0.87%)和1,3,7 - 三甲基 - 2,6 - 二氧嘌呤(0.54%);萜类包括δ - 杜松烯(0.39%)、萜品醇、(+)-雪松醇(22.13%)、3 - 侧柏醇(0.77%)和9,10 - 脱氢环异长叶烯(0.34%);脂肪酸包括顺式 - vaccenic酸(4.98%)和特法酸(1.10%);酯类包括1,2,3 - 丙三醇二乙酸酯(7.56%)、7 - 十六碳烯酸甲酯(1.77%)、二十烷酸甲酯(0.95%);甘油包括1,2,3 - 丙三醇(28.75%)。在MESA中发现的有趣化合物包括还原糖如D - 阿洛糖(4.40%)和连苯三酚(10.48%)、生物碱如咖啡因(63.49%)以及酯类如十八烷酸甲酯(0.53%)。两个提取物都显示出相当的抗氧化活性。报道的现有酚类化合物和萜类是植物提取物抗氧化活性的原因。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4605/7671229/e04b53c63e11/turkjchem-44-352-fig001.jpg

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