Qadir Abdul, Aqil Mohd, Ali Athar, Ahmad Farhan J, Ahmad Sayeed, Arif Muhammad, Khan Nausheen
Department of Pharmaceutics, School of Pharmaceutical Education & Research, Jamia Hamdard, New Delhi, India.
Centre for Transgenic Plant Development, Department of Biotechnology, Jamia Hamdard, New Delhi India.
Turk J Chem. 2020 Apr 1;44(2):352-363. doi: 10.3906/kim-1907-5. eCollection 2020.
L. (family Smilacaceae) and L. (family Salicaceae) are plants that have been traditionally used to treat various ailments in Indian and Chinese medicine. A quantitative estimation of the methanolic extracts of these plants was performed by GC-MS analysis to obtain insight into its phytoconstituents responsible for therapeutic action. The antioxidant potential of the methanol extracts of (MESC) and (MESA) were assessed with DPPH by using a UV spectrophotometer at a wavelength of 517 nm. The prevailing compounds found in MESC were lactam sugars including 2,5-dimethyl-2,4-dihydroxy-3(2H)-furanon (1.40%), 1,5-anhydro-6-deoxyhexo-2,3-diulose (4.33%), and alpha-methyl-1-sorboside (1.80%); the two alkaloids found were 1,4-methane-4,4a,5,6,7,8,9,9a-octahydro-10,10-dimethyl cyclohepta[d] pyridazine (0.87%) and 1,3,7-trimethyl-2,6-dioxopurine(0.54%); terpenes included deltacadinene (0.39%), terpineol, (+)-cedrol (22.13%), 3-thujanol (0.77%), and 9,10-dehydro-cycloisolongifolene (0.34%); fatty acids included cis-vaccenic acid (4.98%) and telfairic acid (1.10%); esters included 1,2,3-propanetriol diacetate (7.56%), 7-hexadecenoic acid, methyl ester (1.77%), eicosanoic acid, and methyl ester (0.95%); and glycerol included 1,2,3-propanetriol (28.75%). The interesting compounds found in MESA were reducing sugars like D-allose (4.40%) and pyrogallol (10.48%), alkaloids like caffeine (63.49%), and esters like methyl octadecanoate (0.53%). Both fractions revealed considerable antioxidant activity. The reported existing phenolic compounds and terpenes are responsible for the antioxidant activity of the plant extracts.
菝葜属(菝葜科)植物和杨属(杨柳科)植物在印度和中国传统医学中一直被用于治疗各种疾病。通过气相色谱 - 质谱联用(GC - MS)分析对这些植物的甲醇提取物进行定量评估,以深入了解其具有治疗作用的植物成分。使用紫外可见分光光度计在波长517nm下,通过二苯基苦味酰基自由基(DPPH)法评估菝葜甲醇提取物(MESC)和杨属甲醇提取物(MESA)的抗氧化潜力。在MESC中发现的主要化合物包括内酰胺糖,如2,5 - 二甲基 - 2,4 - 二羟基 - 3(2H)-呋喃酮(1.40%)、1,5 - 脱水 - 6 - 脱氧己糖 - 2,3 - 二酮(4.33%)和α - 甲基 - 1 - 山梨糖苷(1.80%);发现的两种生物碱为1,4 - 亚甲基 - 4,4a,5,6,7,8,9,9a - 八氢 - 10,10 - 二甲基环庚并[d]哒嗪(0.87%)和1,3,7 - 三甲基 - 2,6 - 二氧嘌呤(0.54%);萜类包括δ - 杜松烯(0.39%)、萜品醇、(+)-雪松醇(22.13%)、3 - 侧柏醇(0.77%)和9,10 - 脱氢环异长叶烯(0.34%);脂肪酸包括顺式 - vaccenic酸(4.98%)和特法酸(1.10%);酯类包括1,2,3 - 丙三醇二乙酸酯(7.56%)、7 - 十六碳烯酸甲酯(1.77%)、二十烷酸甲酯(0.95%);甘油包括1,2,3 - 丙三醇(28.75%)。在MESA中发现的有趣化合物包括还原糖如D - 阿洛糖(4.40%)和连苯三酚(10.48%)、生物碱如咖啡因(63.49%)以及酯类如十八烷酸甲酯(0.53%)。两个提取物都显示出相当的抗氧化活性。报道的现有酚类化合物和萜类是植物提取物抗氧化活性的原因。