Ecker Joseph R, Geschwind Daniel H, Kriegstein Arnold R, Ngai John, Osten Pavel, Polioudakis Damon, Regev Aviv, Sestan Nenad, Wickersham Ian R, Zeng Hongkui
Genomic Analysis Laboratory and Howard Hughes Medical Institute, Salk Institute for Biological Studies, La Jolla, CA 92037, USA.
Program in Neurogenetics, Departments of Neurology and Human Genetics, David Geffen School of Medicine, University of California, Los Angeles, Los Angeles, CA 90095, USA.
Neuron. 2017 Nov 1;96(3):542-557. doi: 10.1016/j.neuron.2017.10.007.
A comprehensive characterization of neuronal cell types, their distributions, and patterns of connectivity is critical for understanding the properties of neural circuits and how they generate behaviors. Here we review the experiences of the BRAIN Initiative Cell Census Consortium, ten pilot projects funded by the U.S. BRAIN Initiative, in developing, validating, and scaling up emerging genomic and anatomical mapping technologies for creating a complete inventory of neuronal cell types and their connections in multiple species and during development. These projects lay the foundation for a larger and longer-term effort to generate whole-brain cell atlases in species including mice and humans.
全面描述神经元细胞类型、它们的分布以及连接模式，对于理解神经回路的特性以及它们如何产生行为至关重要。在此，我们回顾了美国大脑计划资助的十个试点项目——大脑计划细胞普查联盟，在开发、验证和扩大新兴基因组和解剖图谱技术方面的经验，这些技术用于创建多个物种以及发育过程中神经元细胞类型及其连接的完整清单。这些项目为一项规模更大、为期更长的工作奠定了基础，该工作旨在生成包括小鼠和人类在内的物种的全脑细胞图谱。
