Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China.
Research Center for Analytical Sciences, College of Chemistry, Tianjin Key Laboratory of Biosensing and Molecular Recognition, State Key Laboratory of Medicinal Chemical Biology, Nankai University, Tianjin 300071, China; Collaborative Innovation Center of Chemical Science and Engineering (Tianjin), Tianjin 300071, China.
Anal Chim Acta. 2017 Dec 1;995:91-98. doi: 10.1016/j.aca.2017.09.037. Epub 2017 Sep 28.
A simple fluorescence turn-on sensor has been designed for the highly sensitive detection of glycoproteins on the basis of boronic acid functional polymer capped Mn-doped ZnS quantum dots (QDs@MPS@AAPBA). In the absence of glycoproteins, the fluorescence emission intensity of the QDs@MPS@AAPBA was relatively weaker due to the effective electronic transfer from the QDs to the boron moieties on its surface. While the glycoproteins were introduced into the system, an obvious fluorescence enhancement was observed. It was attributed to the boron moieties covalent binding glycans of the glycoproteins resulting in the electronic transfer process being inhibited. Under the optimal conditions, this fluorescent probe not only could be applied in a wide pH range of 5.0-9.0, but also the binding constants and detection limits of the QDs@MPS@AAPBA for horseradish peroxidase (HRP) and transferrin (TRF) were up to 7.23 × 10 M, 1.53 × 10 M and 1.44 × 10 M, 3.36 × 10 M, respectively. Finally, this proposed method has also been utilized for the TRF determination in serum without any complicated pretreatment and the recovery was in the range of 95.7%-103.0%. As a result, it is promising for application on the glycoproteins detection in complex biological samples.
基于硼酸功能聚合物修饰的 Mn 掺杂 ZnS 量子点（QDs@MPS@AAPBA），设计了一种简单的荧光开启型传感器，用于高灵敏度检测糖蛋白。在没有糖蛋白的情况下，由于量子点表面的硼部分与量子点之间的有效电子转移，QDs@MPS@AAPBA 的荧光发射强度相对较弱。而当糖蛋白被引入体系时，观察到明显的荧光增强。这归因于硼部分与糖蛋白的糖链发生共价结合，从而抑制了电子转移过程。在最佳条件下，该荧光探针不仅可以在 5.0-9.0 的宽 pH 范围内应用，而且 QDs@MPS@AAPBA 对辣根过氧化物酶（HRP）和转铁蛋白（TRF）的结合常数和检测限分别高达 7.23×10^M、1.53×10^M 和 1.44×10^M、3.36×10^M。最后，该方法还用于血清中 TRF 的测定，无需任何复杂的预处理，回收率在 95.7%-103.0%范围内。因此，它有望应用于复杂生物样品中的糖蛋白检测。
