Hołyńska-Iwan Iga, Dziembowska Inga, Smyk Paulina, Lampka Magdalena, Olszewska-Słonina Dorota
Department of Pathobiochemistry and Clinical Chemistry, Faculty of Pharmacy, Collegium Medicum in Bydgoszcz Nicolaus Copernicus University in Torun, Bydgoszcz, Poland.
Skin Pharmacol Physiol. 2018;31(1):19-27. doi: 10.1159/000481689. Epub 2017 Nov 8.
Acute, adverse skin effects to capsaicin can be activated by inhibition of sodium transport not only in nociceptive neurons, but also in keratinocytes. The aim of the current study was to describe and compare immediate (15 s) and prolonged (30 min) effects of capsaicin on epidermal (not neural) sodium transport using a rabbit skin model. Skin fragments (n = 169) were incubated in 4 conditions: undisturbed ion transport (U; n = 48); inhibited sodium transport (INa; n = 34) with amiloride used as sodium transport blocker; long-term irritation by capsaicin with undisturbed ion transport (CAPSA-U; n = 43) and with inhibited sodium transport (CAPSA-INa; n = 35). After 30 min of incubation, a solution of capsaicin was applied directly to the skin fragments. The study demonstrated that sodium transport inhibition eliminated the effects of both immediate and prolonged capsaicin application. The results could be the basis for future research considering selective sodium transport inhibitors for human skin to reduce the side effects of capsaicin, related to activation of sodium channels in keratinocytes.
辣椒素引起的急性不良皮肤效应不仅可通过抑制伤害性神经元中的钠转运来激活，还可通过抑制角质形成细胞中的钠转运来激活。本研究的目的是使用兔皮肤模型描述和比较辣椒素对表皮（而非神经）钠转运的即时（15秒）和长期（30分钟）影响。将皮肤碎片（n = 169）在4种条件下孵育：未受干扰的离子转运（U；n = 48）；用氨氯吡咪作为钠转运阻滞剂抑制钠转运（INa；n = 34）；辣椒素长期刺激且离子转运未受干扰（CAPSA-U；n = 43）以及辣椒素长期刺激且钠转运受抑制（CAPSA-INa；n = 35）。孵育30分钟后，将辣椒素溶液直接应用于皮肤碎片。该研究表明，钠转运抑制消除了即时和长期应用辣椒素的影响。这些结果可能为未来研究提供基础，即考虑使用选择性钠转运抑制剂来减少辣椒素与角质形成细胞中钠通道激活相关的副作用。
