Cennamo Nunzio, Zeni Luigi, Tortora Paolo, Regonesi Maria Elena, Giusti Alessandro, Staiano Maria, D'Auria Sabato, Varriale Antonio
Department of Industrial and Information Engineering, University of Campania "Luigi Vanvitelli", 81031 Aversa, Italy.
Department of Industrial and Information Engineering, University of Campania "Luigi Vanvitelli", 81031 Aversa, Italy.
Talanta. 2018 Feb 1;178:955-961. doi: 10.1016/j.talanta.2017.10.034. Epub 2017 Oct 21.
A novel surface plasmon resonance (SPR) optical fiber biosensor, able to bind perfluorooctanoate and perfluorooctanesulfonate compounds, is presented. In the first step, an ad hoc antibody compound has been designed, produced and tested by ELISA, then, in the second step, the gold surface of a plastic optical fiber sensor has been derivatizated and functionalized with this new bio-receptor, able to bind target analytes with high affinity and selectivity. The experimental data have shown that the developed SPR optical fiber biosensor makes it possible to detect these compounds. One advantage of this approach stems from the possibility to monitor the perfluorinated compounds in the environment exploiting the remote sensing capability offered by the optical fibers. The measurements were performed in laboratory, also exploiting matrices mimicking the real environment. The limit of detection of the assay was 0.21ppb, a value that is lower than the maximum residue limit fixed by the European Union regulations.
本文介绍了一种新型的表面等离子体共振（SPR）光纤生物传感器，它能够结合全氟辛酸和全氟辛烷磺酸化合物。第一步，设计、制备并通过酶联免疫吸附测定（ELISA）测试了一种特制的抗体化合物；然后，在第二步中，用这种新的生物受体对塑料光纤传感器的金表面进行衍生化和功能化，使其能够以高亲和力和选择性结合目标分析物。实验数据表明，所开发的SPR光纤生物传感器能够检测这些化合物。这种方法的一个优点是可以利用光纤提供的遥感能力来监测环境中的全氟化合物。测量在实验室中进行，同时还利用了模拟真实环境的基质。该检测方法的检测限为0.21 ppb，这一数值低于欧盟法规规定的最大残留限量。
