Singh Manjeet, Ramassamy Charles
INRS - Institut Armand Frappier, 531, boul. des Prairies, Laval, Québec, Canada H7V 1B7.
Department of Medical Biology, Faculty of Medicine, Laval University, Québec, Canada G1K 7P4.
J Nutr Sci. 2017 Oct 18;6:e54. doi: 10.1017/jns.2017.48. eCollection 2017.
Canine cognitive dysfunction (CCD) is an age-dependent neurodegenerative condition characterised by changes in decline in learning and memory patterns. The neurodegenerative features of CCD in ageing dogs and cats are similar to human ageing and Alzheimer's disease (AD). Discovering neuroprotective disease-modifying therapies against CCD and AD is a major challenge. Strong evidence supports the role of amyloid β peptide deposition and oxidative stress in the pathophysiology of CCD and AD. In both the human and canine brain, oxidative damage progressively increases with age. Dietary antioxidants from natural sources hold a great promise in halting the progression of CCD and AD. (), an Ayurvedic tonic medicine, also known as 'Indian ginseng' or has a long history of use in memory-enhancing therapy but there is a dearth of studies on its neuroprotective effects. The objective of this study was to investigate whether extract can protect against Aβ peptide- and acrolein-induced toxicity. We demonstrated that treatment with extract significantly protected the human neuroblastoma cell line SK-N-SH against Aβ peptide and acrolein in various cell survival assays. Furthermore, treatment with extract significantly reduced the generation of reactive oxygen species in SK-N-SH cells. Finally, our results showed that extract is also a potent inhibitor of acetylcholinesterase activity. Thus, our initial findings indicate that extract may act as an antioxidant and cholinergic modulator and may have beneficial effects in CCD and AD therapy.
犬认知功能障碍（CCD）是一种与年龄相关的神经退行性疾病，其特征是学习和记忆模式下降。衰老犬猫的CCD神经退行性特征与人类衰老和阿尔茨海默病（AD）相似。发现针对CCD和AD的神经保护疾病修饰疗法是一项重大挑战。有力证据支持淀粉样β肽沉积和氧化应激在CCD和AD病理生理学中的作用。在人类和犬类大脑中，氧化损伤都随着年龄的增长而逐渐增加。天然来源的膳食抗氧化剂在阻止CCD和AD的进展方面具有很大的前景。（），一种阿育吠陀滋补药，也被称为“印度人参”，在记忆增强疗法中有着悠久的使用历史，但关于其神经保护作用的研究却很少。本研究的目的是调查（）提取物是否能预防Aβ肽和丙烯醛诱导的毒性。我们证明，在各种细胞存活试验中，用（）提取物处理可显著保护人神经母细胞瘤细胞系SK-N-SH免受Aβ肽和丙烯醛的损伤。此外，用（）提取物处理可显著减少SK-N-SH细胞中活性氧的产生。最后，我们的结果表明，（）提取物也是乙酰胆碱酯酶活性的有效抑制剂。因此，我们的初步研究结果表明，（）提取物可能作为一种抗氧化剂和胆碱能调节剂，在CCD和AD治疗中可能具有有益作用。
