Elango N, Kövamees J, Varsanyi T M, Norrby E
Department of Virology, School of Medicine, Karolinska Institute, Stockholm, Sweden.
J Virol. 1989 Mar;63(3):1413-5. doi: 10.1128/JVI.63.3.1413-1415.1989.
The mRNA of a putative small hydrophobic protein (SH) of mumps virus was identified in mumps virus-infected Vero cells, and its complete nucleotide sequence was determined by sequencing the genomic RNA and cDNA clones and partial sequencing of mRNA. The SH mRNA is 310 nucleotides long excluding the poly(A) and contains a single open reading frame encoding a protein of 57 amino acids with a calculated molecular weight of 6,719. The predicted protein is highly hydrophobic and contains a stretch of 25 hydrophobic amino acids near the amino terminus which could act as a membrane anchor region. There is no homology between the putative SH protein of mumps virus and the SH protein of simian virus 5, even though the SH genes are located in the same locus in the corresponding genome. One interesting observation is that the hydrophobic domain of simian virus 5 SH protein is at the carboxyl terminus, whereas that of mumps virus putative SH protein is near the amino terminus.
在腮腺炎病毒感染的非洲绿猴肾细胞（Vero细胞）中鉴定出了一种假定的腮腺炎病毒小疏水蛋白（SH）的信使核糖核酸（mRNA），通过对基因组核糖核酸（RNA）和互补脱氧核糖核酸（cDNA）克隆进行测序以及对mRNA进行部分测序，确定了其完整的核苷酸序列。SH mRNA长310个核苷酸（不包括聚腺苷酸尾），包含一个单一的开放阅读框，编码一个由57个氨基酸组成的蛋白质，计算分子量为6719。预测的蛋白质具有高度疏水性，在氨基末端附近有一段25个疏水氨基酸的序列，可作为膜锚定区域。尽管SH基因位于相应基因组的同一位置，但腮腺炎病毒假定的SH蛋白与猿猴病毒5的SH蛋白之间没有同源性。一个有趣的发现是，猿猴病毒5 SH蛋白的疏水结构域在羧基末端，而腮腺炎病毒假定的SH蛋白的疏水结构域在氨基末端附近。
