Mohanta Yugal Kishore, Biswas Kunal, Panda Sujogya Kumar, Bandyopadhyay Jaya, De Debashis, Jayabalan Rasu, Bastia Akshaya Kumar, Mohanta Tapan Kumar
Biochemistry Laboratory, Department of Botany, North Orissa University, Baripada, Odisha 757 003, India.
Department of Biotechnology, Maulana Abul Kalam Azad University of Technology, West Bengal, Kolkata 700 064, India.
IET Nanobiotechnol. 2017 Dec;11(8):1027-1034. doi: 10.1049/iet-nbt.2017.0017.
Bio- synthesis of silver nanoparticles (AgNPs) was made by using the aqueous leaf extract of Ardisia solanacea. Rapid formation of AgNPs was observed from silver nitrate upon treatment with the aqueous extract of A. solanacea leaf. The formation and stability of the AgNPs in the colloidal solution were monitored by UV-visible spectrophotometer. The mean particle diameter of AgNPs was calculated from the DLS with an average size ∼4 nm and ∼65 nm. ATR-FTIR spectroscopy confirmed the presence of alcohols, aldehydes, flavonoids, phenols and nitro compounds in the leaf which act as the stabilizing agent. Antimicrobial activity of the synthesized AgNPs was performed using agar well diffusion and broth dilution method against the Gram-positive and Gram-negative bacteria. Further, robust anti-oxidative potential was evaluated by DPPH assay. The highest antimicrobial activity of synthesized AgNPs was found against Pseudomonas aeruginosa (28.2 ± 0.52 mm) whereas moderate activity was found against Bacillus subtilis (16.1 ± 0.76), Candida kruseii (13.0 ± 1.0), and Trichophyton mentagrophytes (12.6 ± 1.52). Moreover, the potential wound healing activity was observed against the BJ-5Ta normal fibroblast cell line. Current research revealed that A. solanacea was found to be a suitable source for the green synthesis of silver nanoparticles.
通过使用茄叶紫金牛的水提叶提取物进行银纳米颗粒（AgNPs）的生物合成。在用茄叶紫金牛叶水提取物处理后，观察到硝酸银迅速形成AgNPs。通过紫外可见分光光度计监测胶体溶液中AgNPs的形成和稳定性。通过动态光散射（DLS）计算AgNPs的平均粒径，平均尺寸约为4 nm和65 nm。衰减全反射傅里叶变换红外光谱（ATR-FTIR）证实叶片中存在醇类、醛类、黄酮类、酚类和硝基化合物，它们作为稳定剂。使用琼脂扩散法和肉汤稀释法对合成的AgNPs对革兰氏阳性和革兰氏阴性细菌进行抗菌活性测试。此外，通过1,1-二苯基-2-苦基肼（DPPH）测定评估其强大的抗氧化潜力。发现合成的AgNPs对铜绿假单胞菌的抗菌活性最高（28.2 ± 0.52 mm），而对枯草芽孢杆菌（16.1 ± 0.76）、克鲁斯念珠菌（13.0 ± 1.0）和须癣毛癣菌（12.6 ± 1.52）的活性中等。此外，观察到对BJ-5Ta正常成纤维细胞系具有潜在的伤口愈合活性。当前研究表明，茄叶紫金牛被发现是绿色合成银纳米颗粒的合适来源。
