Hussain Arif, Shaik Sabiha, Ranjan Amit, Nandanwar Nishant, Tiwari Sumeet K, Majid Mohammad, Baddam Ramani, Qureshi Insaf A, Semmler Torsten, Wieler Lothar H, Islam Mohammad A, Chakravortty Dipshikha, Ahmed Niyaz
Department of Microbiology and Cell Biology, Indian Institute of Science, Bengaluru, India.
Pathogen Biology Laboratory, Department of Biotechnology and Bioinformatics, University of Hyderabad, Hyderabad, India.
Front Microbiol. 2017 Nov 13;8:2120. doi: 10.3389/fmicb.2017.02120. eCollection 2017.
Multidrug-resistant infections are a growing public health concern. This study analyzed the possibility of contamination of commercial poultry meat (broiler and free-range) with pathogenic and or multi-resistant in retail chain poultry meat markets in India. We analyzed 168 isolates from broiler and free-range retail poultry (meat/ceca) sampled over a wide geographical area, for their antimicrobial sensitivity, phylogenetic groupings, virulence determinants, extended-spectrum-β-lactamase (ESBL) genotypes, fingerprinting by Enterobacterial Repetitive Intergenic Consensus (ERIC) PCR and genetic relatedness to human pathogenic using whole genome sequencing (WGS). The prevalence rates of ESBL producing among broiler chicken were: meat 46%; ceca 40%. Whereas, those for free range chicken were: meat 15%; ceca 30%. from broiler and free-range chicken exhibited varied prevalence rates for multi-drug resistance (meat 68%; ceca 64% and meat 8%; ceca 26%, respectively) and extraintestinal pathogenic (ExPEC) contamination (5 and 0%, respectively). WGS analysis confirmed two globally emergent human pathogenic lineages of , namely the ST131 (30-Rx subclone) and ST117 among our poultry isolates. These results suggest that commercial poultry meat is not only an indirect public health risk by being a possible carrier of non-pathogenic multi-drug resistant (MDR)-, but could as well be the carrier of human pathotypes. Further, the free-range chicken appears to carry low risk of contamination with antimicrobial resistant and extraintestinal pathogenic (ExPEC). Overall, these observations reinforce the understanding that poultry meat in the retail chain could possibly be contaminated by MDR and/or pathogenic
多重耐药感染正日益引起公共卫生关注。本研究分析了印度零售连锁禽肉市场上商业家禽肉（肉鸡和散养鸡）被致病性和/或多重耐药菌污染的可能性。我们分析了从广泛地理区域采集的168株来自肉鸡和散养零售家禽（肉/盲肠）的分离株，检测其抗菌敏感性、系统发育分组、毒力决定因素、超广谱β-内酰胺酶（ESBL）基因型、通过肠杆菌重复基因间共识（ERIC）PCR进行指纹图谱分析以及使用全基因组测序（WGS）分析与人类致病菌株的遗传相关性。肉鸡中产ESBL菌株的流行率为：肉46%；盲肠40%。而散养鸡的流行率为：肉15%；盲肠30%。肉鸡和散养鸡的分离株在多重耐药（分别为肉68%；盲肠64%和肉8%；盲肠26%）和肠外致病性大肠杆菌（ExPEC）污染（分别为5%和0%）方面表现出不同的流行率。WGS分析证实了我们家禽分离株中存在两种全球出现的人类致病大肠杆菌谱系，即ST131（30-Rx亚克隆）和ST117。这些结果表明，商业家禽肉不仅因其可能是非致病性多重耐药大肠杆菌（MDR）的携带者而构成间接公共卫生风险，还可能是人类致病型大肠杆菌的携带者。此外，散养鸡似乎携带抗菌耐药和肠外致病性大肠杆菌（ExPEC）污染的低风险。总体而言，这些观察结果强化了这样的认识，即零售连锁中的家禽肉可能被MDR和/或致病性大肠杆菌污染
