Tsai Meng-Tsan, Chen Yingdan, Lee Cheng-Yu, Huang Bo-Huei, Trung Nguyen Hoang, Lee Ya-Ju, Wang Yen-Li
Department of Electrical Engineering, Chang Gung University, Taoyuan, 33302 Taiwan.
Medical Imaging Research Center, Institute for Radiological Research, Chang Gung University and Chang Gung Memorial Hospital at Linkou, Taoyuan, 33302 Taiwan.
Biomed Opt Express. 2017 Oct 13;8(11):5001-5012. doi: 10.1364/BOE.8.005001. eCollection 2017 Nov 1.
In this study, we demonstrated the feasibility of using a handheld optical coherence tomography (OCT) for visualizations of the microstructural and microvascular features of various oral mucosal types. To scan arbitrary locations of the oral mucosa, a scanning probe was developed, composed of a probe body fabricated by a 3D printer, miniaturized two-axis galvanometer, relay lenses, and reflective prism. With a 3D printing technique, the probe weight and the system volume were greatly reduced, enabling the effective improvement of imaging artifacts from unconscious motion and system complexity. Additionally, in our design, the distal end of the probe can be switched to fit various oral conditions, and the optical parameters of the probe, such as the transverse resolution, working distance, and probe length can be easily varied. The results showed that the epithelium and lamina propria layers, as well as the fungiform papilla and salivary gland, were differentiated. Moreover, various microcirculation features at different mucosal sites were identified that are potentially effective indicators for the diagnosis of premalignant lesions. The demonstrated results indicate that the developed OCT system is a promising tool for noninvasive imaging of oral mucosae.
在本研究中，我们证明了使用手持式光学相干断层扫描（OCT）可视化各种口腔黏膜类型的微观结构和微血管特征的可行性。为了扫描口腔黏膜的任意位置，开发了一种扫描探头，它由3D打印机制造的探头主体、小型化的两轴振镜、中继透镜和反射棱镜组成。通过3D打印技术，探头重量和系统体积大大减小，有效减少了因无意识运动和系统复杂性产生的成像伪影。此外，在我们的设计中，探头远端可以切换以适应各种口腔状况，并且探头的光学参数，如横向分辨率、工作距离和探头长度可以很容易地改变。结果表明，上皮层和固有层以及菌状乳头和唾液腺都能被区分出来。此外，还识别出不同黏膜部位的各种微循环特征，这些特征可能是诊断癌前病变的有效指标。所展示的结果表明，所开发的OCT系统是一种用于口腔黏膜无创成像的有前途的工具。
