Arshed Muhammad Jefte C, Valdez Marcos B, Alejandro Grecebio Jonathan D
The Graduate School, University of Santo Tomas, España Blvd., 1015 Manila, Philippines.
Department of Biological Sciences, Institute of Arts and Sciences, Far Eastern University, Nicanor Reyes Street, Sampaloc, 1008 Manila, Philippines.
Pharmacogn Mag. 2017 Oct-Dec;13(52):553-558. doi: 10.4103/pm.pm_1_17. Epub 2017 Nov 13.
The pantropical genus Jack is identified for high phenotypic plasticity making traditional taxonomic identification difficult. Having some members with important medicinal properties, a precise complimentary identification through DNA barcoding is needed for species delineation.
In this study, 12 samples representing six Philippine species were used to determine the most efficient barcoding loci among the cpDNA markers (K, L, 16, and T-F) and nrDNA (ITS) based on the criteria of universality, discriminatory power, and resolution of species.
The results revealed that ITS has the recommended primer universality, greatest interspecific divergences, and average resolution of species. Among the cpDNA markers, K and L are recommended but with minimal resolution of species. While T-F showed moderate interspecific variations and resolution of species, 16 has the lowest interspecific divergence and resolution of species.
Consequently, ITS is the potential ideal DNA barcode for species.
ITS, K, and 16 markers have the excellent amplification and sequence qualityITS marker has the highest interspecific divergence with the maximum values, followed by K, L, T-F, and 16, respectivelyAll markers except 16 yielded average resolution to Lasianthus speciesITS marker is the most ideal locus in terms of excellent universality, high interspecific discriminatory ability, and average species resolution. ITS: Internal Transcribe Spacer, K: maturase K, L: ribulose-1,5-biphospahte-carboxylase, 16: ribosomal protein 16 small subunit gene.
泛热带的紫金牛属植物具有高度的表型可塑性，这使得传统的分类鉴定变得困难。该属的一些成员具有重要的药用特性，因此需要通过DNA条形码进行精确的补充鉴定以进行物种划分。
在本研究中，使用了代表六个菲律宾物种的12个样本，根据通用性、鉴别力和物种分辨率标准，确定叶绿体DNA标记（K、L、16和T-F）和核核糖体DNA（ITS）中最有效的条形码位点。
结果表明，ITS具有推荐的引物通用性、最大的种间差异和平均物种分辨率。在叶绿体DNA标记中，推荐使用K和L，但物种分辨率最低。虽然T-F显示出中等的种间变异和物种分辨率，16的种间差异和物种分辨率最低。
因此，ITS是潜在的理想物种DNA条形码。
ITS、K和16标记具有出色的扩增和序列质量。ITS标记的种间差异最大，其次分别是K、L、T-F和16。除16外，所有标记对粗叶木属物种的分辨率均为中等。ITS标记在通用性、种间鉴别能力和物种分辨率方面是最理想的位点。ITS：内转录间隔区，K：成熟酶K，L：核酮糖-1,5-二磷酸羧化酶，16：核糖体蛋白小亚基16基因
