Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education (Northwest University), Provincial Key Laboratory of Biotechnology (Northwest University), College of Life Sciences, Northwest University, Xi'an 710069, China.
Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education (Northwest University), Provincial Key Laboratory of Biotechnology (Northwest University), College of Life Sciences, Northwest University, Xi'an 710069, China.
Int J Biol Macromol. 2018 Apr 1;109:611-618. doi: 10.1016/j.ijbiomac.2017.12.017. Epub 2017 Dec 6.
Traditional separation and purification process of Lycium barbarum polysaccharides (LBP) includes water extraction, alcohol precipitation, deproteinization and ion-exchange column chromatography, which is complicated and time-consuming. In our study, retentate LBP-I and dialysate LBP-O were obtained from LBP by water extraction, alcohol precipitation and deproteinization. LBP-I was separated by fractional precipitation and three fractions (LBP-I-1, LBP-I-2 and LBP-I-3) were obtained. The three fractions were further purified by gel permeation chromatography to LBGP-I-1, LBGP-I-2 and LBGP-I-3 with yields of 0.05%, 0.03%, and 0.19%, respectively, which are higher than yields by traditional method. The physicochemical properties, biological activities of LBGP-I-1, LBGP-I-2 and LBGP-I-3 were investigated. The results indicated that LBGP-I-1 (3.19 × 10 Da) consists of arabinose (21.95%), glucose (51.22%) and galactose (17.07%); LBGP-I-2 (2.92 × 10 Da) mainly consists of arabinose (19.35%), glucose (32.26%) and galactose (35.48%); LBGP-I-3 (9.12 × 10 Da) mainly consists of arabinose (48.15%) and galactose (44.44%). LBGP-I-1 and LBGP-I-2 were different from the components purified by traditional method. LBGP-I-3 could most significantly enhance macrophages NO, phagocytic capacity, and acid phosphatase. LBP-O exhibits the strongest anti-oxidant activities in vitro. These results provided a reference for applications of Lycium barbarum polysaccharides which would benefit the development of industry and agriculture.
传统的枸杞多糖（LBP）分离纯化工艺包括水提、醇沉、脱蛋白和离子交换柱层析，过程复杂且耗时。本研究采用水提法、醇沉法和脱蛋白法从枸杞中提取得到浓缩液 LBP-I 和透析液 LBP-O。LBP-I 经分步沉淀法分离得到 3 个馏分（LBP-I-1、LBP-I-2 和 LBP-I-3）。将这 3 个馏分进一步通过凝胶渗透色谱法纯化，得到 LBGP-I-1、LBGP-I-2 和 LBGP-I-3，收率分别为 0.05%、0.03%和 0.19%，均高于传统方法的收率。对 LBGP-I-1、LBGP-I-2 和 LBGP-I-3 的理化性质和生物活性进行了研究。结果表明，LBGP-I-1（3.19×10 Da）由阿拉伯糖（21.95%）、葡萄糖（51.22%）和半乳糖（17.07%）组成；LBGP-I-2（2.92×10 Da）主要由阿拉伯糖（19.35%）、葡萄糖（32.26%）和半乳糖（35.48%）组成；LBGP-I-3（9.12×10 Da）主要由阿拉伯糖（48.15%）和半乳糖（44.44%）组成。LBGP-I-1 和 LBGP-I-2 的成分与传统方法分离得到的成分不同。LBGP-I-3 能最显著地增强巨噬细胞 NO、吞噬能力和酸性磷酸酶的产生。LBP-O 具有最强的体外抗氧化活性。这些结果为枸杞多糖的应用提供了参考，有利于工农业的发展。
