Ianchis Raluca, Ninciuleanu Claudia M, Gifu Ioana C, Alexandrescu Elvira, Somoghi Raluca, Gabor Augusta R, Preda Silviu, Nistor Cristina L, Nitu Sabina, Petcu Cristian, Icriverzi Madalina, Florian Paula E, Roseanu Anca M
National R-D Institute for Chemistry and Petrochemistry ICECHIM-Bucharest, Spl. Independentei 202, 6th district, P.O. Box 35/174, 060021 Bucharest, Romania.
Institute of Physical Chemistry "Ilie Murgulescu", Romanian Academy, Spl. Independentei 202, 6th district, P.O. Box 194, 060021 Bucharest, Romania.
Nanomaterials (Basel). 2017 Dec 13;7(12):443. doi: 10.3390/nano7120443.
Present study refers to the synthesis of new advanced materials based on poly(methacrylic acid) (PMAA) with previously reported own advanced modified clays by edge covalent bonding. This will create the premises to obtain nanocomposite hydrogels with combined hydrophilic-hydrophobic behavior absolutely necessary for co-delivery of polar/nonpolar substances. For the synthesis, ,'-methylenebisacrylamide was used as cross-linker and ammonium persulphate as initiator. As a consequence of the inclusion of clay into the polymer matrix and the intercalation of PMAA between the layers as well as the presence of hydrophobic interactions occurred between partners, the final hydrogel nanocomposites possessed greater swelling degrees, slower de-swelling process and enhanced mechanical properties depending on the clay type in comparison with pure hydrogel. In vitro MTS ([3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2-tetrazolium, inner salt]) colorimetric assay showed that direct exposure with PMMA-clay-based constructs did not affect cell viability and proliferation in time (24 and 48 h) on either normal or adenocarcinoma cell lines.
本研究涉及基于聚（甲基丙烯酸）（PMAA）与先前报道的通过边缘共价键合的自身先进改性粘土合成新型先进材料。这将为获得具有极性/非极性物质共递送绝对必需的亲水-疏水组合行为的纳米复合水凝胶创造前提条件。在合成过程中，使用N,N'-亚甲基双丙烯酰胺作为交联剂，过硫酸铵作为引发剂。由于将粘土纳入聚合物基质、PMAA插入层间以及伙伴之间存在疏水相互作用，与纯水凝胶相比，最终的水凝胶纳米复合材料根据粘土类型具有更大的溶胀度、更慢的去溶胀过程和增强的机械性能。体外MTS（[3-(4,5-二甲基噻唑-2-基)-5-(3-羧基甲氧基苯基)-2-(4-磺基苯基)-2-四唑鎓，内盐]）比色法显示，与基于PMMA-粘土的构建体直接接触在24小时和48小时内对正常或腺癌细胞系的细胞活力和增殖均无影响。
