Lu Qian, Hao Meng, Wu Wenya, Zhang Nan, Isaac Adelusi Temitope, Yin Jiale, Zhu Xia, Du Lei, Yin Xiaoxing
Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical College, Xuzhou 221004 China.
Acta Biochim Pol. 2018;65(1):35-41. doi: 10.18388/abp.2016_1387. Epub 2017 Dec 28.
One of the earliest critical secondary complications of diabetes is the opacification of the eye lens - a condition strictly associated with diabetic cataract. The study presented here was designed to investigate the effect of Ginkgo biloba extract (GbE), rutin and quercetin on streptozotocin (STZ) induced diabetic cataract (DC) rats. Ten weeks after administration of GbE, rutin and quercetin, the opacity of diabetic rats' lenses was graded under a slit lamp. Then, the levels of malondialdehyde (MDA), reduced glutathione (GSH), advanced glycosylation end products (AGEs), and the activities of aldose reductase (AR) were estimated. The DC-induced rats produced less GSH, higher levels of MDA and AGEs as well as elevated AR activity when compared to the normal group. Administration of GbE, rutin and quercetin remarkably inhibited the AR activity, stimulated the production of glutathione, and decreased the levels of MDA and AGEs in the lenses of DC-induced rats, which eventually delayed the progression of lens opacification in diabetic rats to various degrees. Our results revealed that quercetin had the highest significant (P<0.05) potential to delay the progression of STZ-induced diabetic cataract when compared with rutin and GbE. The mechanism dictating this interesting prowess of quercetin might be attributed to its AR inhibitory strength, anti-lipid peroxidation potential and anti-AGEs activity.
糖尿病最早出现的严重继发性并发症之一是晶状体混浊——一种与糖尿病性白内障密切相关的病症。本文所呈现的研究旨在探究银杏叶提取物（GbE）、芦丁和槲皮素对链脲佐菌素（STZ）诱导的糖尿病性白内障（DC）大鼠的影响。在给予GbE、芦丁和槲皮素10周后，在裂隙灯下对糖尿病大鼠晶状体的混浊程度进行分级。然后，测定丙二醛（MDA）、还原型谷胱甘肽（GSH）、晚期糖基化终产物（AGEs）的水平以及醛糖还原酶（AR）的活性。与正常组相比，诱导糖尿病的大鼠产生的GSH较少，MDA和AGEs水平较高，且AR活性升高。给予GbE、芦丁和槲皮素可显著抑制AR活性，刺激谷胱甘肽的产生，并降低诱导糖尿病大鼠晶状体中MDA和AGEs的水平，最终不同程度地延缓了糖尿病大鼠晶状体混浊的进展。我们的结果显示，与芦丁和GbE相比，槲皮素在延缓STZ诱导的糖尿病性白内障进展方面具有最高的显著（P<0.05）潜力。槲皮素这种有趣功效的作用机制可能归因于其AR抑制强度、抗脂质过氧化潜力和抗AGEs活性。
