不同阶段子宫内膜异位症患者血清和腹腔液中炎性细胞因子的表达

Expression of inflammatory cytokines in serum and peritoneal fluid from patients with different stages of endometriosis.

作者信息

Fan Yan-Yan, Chen Hong-Yu, Chen Wei, Liu Yi-Nan, Fu Yan, Wang Li-Na

机构信息

a Department of Obstetrics and Gynecology , The First Hospital of Jilin University , Changchun , China.

b Peking University of Medical Department , Beijing , China.

出版信息

Gynecol Endocrinol. 2018 Jun;34(6):507-512. doi: 10.1080/09513590.2017.1409717. Epub 2018 Jan 8.

DOI:10.1080/09513590.2017.1409717

PMID:29308924

Abstract

OBJECTIVE

To investigate the level of inflammatory cytokines in endometriosis patients, and explore the relationship between IL-37 concentration and endometriosis stages.

METHODS

Inflammatory cytokine concentrations from 27 patients with different stages of endometriosis and 52 controls without endometriosis were examined by ELISA. Then, the specificity and sensitivity of cytokines for distinguishing from controls and the different stages of endometriosis were analyzed using the ROC curve.

RESULTS

The difference in serum concentrations of IL-37, IL-17A, IL-10, and IL-2 between the endometriosis and control groups was statistically significant (p < .01). Compared with controls, significantly higher levels of serum IL-37 and IL-10, and significantly lower levels of serum IL-17A and IL-2 were detected in patients with endometriosis (p < .01). Furthermore, IL-2 concentration was significantly higher in peritoneal fluid (PF) in the endometriosis group (p = .0034), IL-10 concentrations in PF were significantly lower in the early-stages of endometriosis than in the more advanced groups (p = .0439), and IL-4 concentration in PF was significantly higher in more advanced endometriosis (p = .0228). The sensitivity and specificity of serum IL-37 for distinguishing endometriosis were 81.48% and 83.33%, respectively, and the cutoff concentration was 69.84 pg/ml. For IL-17A, the sensitivity and specificity were 96.30% and 100%, respectively, and the cutoff concentration was 57.54 pg/ml. For IL-10, the sensitivity and specificity was 92.59% and 100%, respectively, and the cutoff concentration was 3.301 pg/ml. For IL-2, the sensitivity and specificity were 74.07% and 93.75%, respectively, and the cutoff concentration was 1.813 pg/ml. For PF IL-2, the sensitivity and specificity were 29.73% and 100%, respectively, and the cutoff concentration was 1.06 pg/ml.

CONCLUSIONS

IL-37, IL-17A, IL-10, and IL-2 may play a significant role in immune response in endometriosis. IL-37 levels may be used as a diagnostic marker for endometriosis.

摘要

目的

研究子宫内膜异位症患者炎症细胞因子水平，探讨白细胞介素-37（IL-37）浓度与子宫内膜异位症分期的关系。

方法

采用酶联免疫吸附测定法（ELISA）检测27例不同分期子宫内膜异位症患者及52例非子宫内膜异位症对照者的炎症细胞因子浓度。然后，利用ROC曲线分析细胞因子区分对照者与子宫内膜异位症不同分期的特异性和敏感性。

结果

子宫内膜异位症组与对照组血清中IL-37、IL-17A、IL-10和IL-2浓度差异有统计学意义（p < 0.01）。与对照组相比，子宫内膜异位症患者血清IL-37和IL-10水平显著升高，血清IL-17A和IL-2水平显著降低（p < 0.01）。此外，子宫内膜异位症组腹腔液（PF）中IL-2浓度显著升高（p = 0.0034），子宫内膜异位症早期PF中IL-10浓度显著低于进展期组（p = 0.0439），进展期子宫内膜异位症PF中IL-4浓度显著升高（p = 0.0228）。血清IL-37区分子宫内膜异位症的敏感性和特异性分别为81.48%和83.33%，截断浓度为69.84 pg/ml。IL-17A的敏感性和特异性分别为96.30%和100%，截断浓度为57.54 pg/ml。IL-10的敏感性和特异性分别为92.59%和100%，截断浓度为3.301 pg/ml。IL-2的敏感性和特异性分别为74.07%和93.75%，截断浓度为1.813 pg/ml。PF中IL-2的敏感性和特异性分别为29.73%和100%，截断浓度为1.06 pg/ml。