Kaur Rupinder, Kaur Sukhbir
Parasitology Laboratory, Department of Zoology, Panjab University, Chandigarh-160014, India.
J Tradit Complement Med. 2017 Jun 27;8(1):251-260. doi: 10.1016/j.jtcme.2017.06.006. eCollection 2018 Jan.
Medicinal plants with immunomodulatory properties can provide good alternative therapeutics for curing visceral leishmaniasis. (Wall.) Engl. is an interesting plant with strong antioxidant, antimicrobial, immunomodulatory and hepatoprotective properties.
The present study was planned to determine the antileishmanial activity of plant extract by modulating the immune responses of inbred BALB/c mice.
Bergenin, the principle active component of , was quantitated in crude extract by performing RP-HPLC. The therapeutic potential was assessed through antileishmanial activity and in mice model through parasite load, cytokine assays, IgG antibody levels, DTH responses, histopathology and biochemical enzyme assays.
showed the presence of glycosides, saponins, carbohydrates, tannins, flavonoids and bergenin which contributed to the antileishmanial activity of extract with IC50 of 22.70 μg/mL. Furthermore, the higher dose significantly reduced the parasite load by 95.56 %. The reduction was further associated with significant enhancement of IL-12 and IFN-γ levels in comparison to IL-10 and IL-4 cytokines. The switching towards Th1 type of immune response was also confirmed by elevated antibody levels of IgG2a isotype as compared to IgG1 as well as increased DTH responses. The histology of liver and kidney further complimented the non toxic nature of plant extract in addition to its negligible toxicity on HeLa cells.
The current study revealed the significant antileishmanial and immunomodulatory properties of this plant extract against murine visceral leishmaniasis. Further, the bioactive components will be explored to assess their efficacy for the development of safe and cost effective drug.
具有免疫调节特性的药用植物可为治疗内脏利什曼病提供良好的替代疗法。（Wall.） Engl. 是一种具有强大抗氧化、抗菌、免疫调节和保肝特性的有趣植物。
本研究旨在通过调节近交系BALB/c小鼠的免疫反应来确定植物提取物的抗利什曼活性。
通过RP-HPLC对粗提物中的主要活性成分岩白菜素进行定量。通过抗利什曼活性评估其治疗潜力，并在小鼠模型中通过寄生虫负荷、细胞因子检测、IgG抗体水平、迟发型超敏反应（DTH）、组织病理学和生化酶检测进行评估。
显示存在糖苷、皂苷、碳水化合物、单宁、黄酮类化合物和岩白菜素，这些成分有助于提取物的抗利什曼活性，IC50为22.70μg/mL。此外，较高剂量显著降低了95.56%的寄生虫负荷。与IL-10和IL-4细胞因子相比，这种降低还与IL-12和IFN-γ水平的显著提高有关。与IgG1相比，IgG2a同种型抗体水平升高以及DTH反应增加也证实了向Th1型免疫反应的转变。肝脏和肾脏的组织学进一步证实了植物提取物的无毒性质，以及其对HeLa细胞的可忽略不计的毒性。
当前研究揭示了这种植物提取物对小鼠内脏利什曼病具有显著的抗利什曼和免疫调节特性。此外，将探索生物活性成分以评估它们在开发安全且经济有效的药物方面的功效。
