School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
School of Pharmacy, Jiangsu University, Zhenjiang 212013, China.
Chin J Nat Med. 2017 Dec;15(12):905-911. doi: 10.1016/S1875-5364(18)30006-2.
The present study was designed to explore the influence of water extracts of Atractylodes lancea rhizomes on the toxicity and anti-inflammatory effects of triptolide (TP). A water extract was prepared from A. lancea rhizomes and co-administered with TP in C57BL/6 mice. The toxicity was assayed by determining serum biochemical parameters and visceral indexes and by liver histopathological analysis. The hepatic CYP3A expression levels were detected using Western blotting and RT-PCR methods. The data showed that the water extract of A. lancea rhizomes reduced triptolide-induced toxicity, probably by inducing the hepatic expression of CYP3A. The anti-inflammatory effects of TP were evaluated in mice using a xylene-induced ear edema test. By comparing ear edema inhibition rates, we found that the water extract could also increase the anti-inflammatory effects of TP. In conclusion, our results suggested that the water extract of A. lancea rhizomes, used in combination with TP, has a potential in reducing TP-induced toxicity and enhancing its anti-inflammatory effects.
本研究旨在探讨白术水提取物对雷公藤红素（TP）毒性和抗炎作用的影响。采用白术根茎水提取物与 TP 联合给药，对 C57BL/6 小鼠进行毒性检测，通过血清生化参数和内脏指数以及肝组织病理分析来评估毒性。采用 Western blot 和 RT-PCR 方法检测肝 CYP3A 表达水平。结果表明，白术根茎水提取物可降低雷公藤红素诱导的毒性，可能是通过诱导肝 CYP3A 的表达。采用二甲苯诱导的耳肿胀试验评估 TP 的抗炎作用。通过比较耳肿胀抑制率，发现水提取物还可以增强 TP 的抗炎作用。综上所述，本研究结果提示，白术根茎水提取物与 TP 联合应用具有降低 TP 诱导的毒性和增强其抗炎作用的潜力。
