Wang Xinxin, Peng Jingyu, Sun Lei, Bonito Gregory, Guo Yuxiu, Li Yu, Fu Yongping
Department of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China.
Engineering Research Center of Edible and Medicinal Fungi, Ministry of Education, Jilin Agricultural University, Changchun 130118, China.
Pathogens. 2020 Oct 13;9(10):834. doi: 10.3390/pathogens9100834.
Morels ( spp.) are popular edible fungi with significant economic and scientific value. However, white mold disease, caused by , can reduce morel yield by up to 80% in the main cultivation area in China. is a polyphyletic genus and the exact phylogenetic placement of is currently still unclear. Here, we obtained the first high-quality genome sequence of generated through the single-molecule real-time (SMRT) sequencing platform. The assembled draft genome of was 40.2 Mb, had an N50 value of 2.6 Mb and encoded 9454 genes. Phylogenetic analysis of single-copy orthologous genes revealed that is in Hypocreales and closely related to Hypocreaceae, which includes several genera exhibiting a mycoparasitic lifestyle. CAZymes analysis demonstrated that encodes a large number of fungal cell wall degradation enzymes. We identified many gene clusters involved in the production of secondary metabolites known to exhibit antifungal, antibacterial, or insecticidal activities. We further demonstrated through dual culture assays that secretes certain soluble compounds that are inhibitory to the mycelial growth of . This study provides insights into the correct phylogenetic placement of and the molecular mechanisms that underlie pathogenesis.
羊肚菌（羊肚菌属）是具有重要经济和科学价值的受欢迎的可食用真菌。然而，由[未提及的病原体]引起的白霉病在中国主要种植区可使羊肚菌产量降低多达80%。羊肚菌是一个多系属，目前其确切的系统发育位置仍不清楚。在此，我们通过单分子实时（SMRT）测序平台获得了首个高质量的羊肚菌基因组序列。组装后的羊肚菌基因组草图为40.2 Mb，N50值为2.6 Mb，编码9454个基因。对单拷贝直系同源基因的系统发育分析表明，羊肚菌属于肉座菌目，与肉座菌科密切相关，肉座菌科包括几个表现出真菌寄生生活方式的属。碳水化合物活性酶分析表明，羊肚菌编码大量真菌细胞壁降解酶。我们鉴定出许多参与已知具有抗真菌、抗菌或杀虫活性的次生代谢产物产生的基因簇。我们通过共培养试验进一步证明，羊肚菌分泌某些可抑制[未提及的病原体]菌丝生长的可溶性化合物。本研究为羊肚菌的正确系统发育位置以及羊肚菌发病机制的分子基础提供了见解。
