Zhang Qian, Cong Renhuai, Hu Minghua, Zhu Yanhong, Yang Xiangliang
College of Life Science and Technology, Huazhong University of Science and Technology, Wuhan, China.
Joint Laboratory for the Research of Pharmaceutics, Huazhong University of Science and Technology and Infinitus, Wuhan, China.
Evid Based Complement Alternat Med. 2017;2017:9459156. doi: 10.1155/2017/9459156. Epub 2017 Nov 20.
Fungal polysaccharides display a variety of important biological activities, including anti-inflammatory, antitumor, and immune-stimulating activities. The aim of present study was to investigate the immunomodulatory effect of fungal polysaccharides on cyclophosphamide-induced immunosuppression in mice. Mice were pretreated orally with lentinan, tremellan, pachymaran, or a mixture of the three, respectively. The results showed that pretreatments with polysaccharides significantly increased the thymus index in cyclophosphamide-induced immunosuppression mice. The level of the cytokine IL-10 in sera of cyclophosphamide-induced mice was decreased after pretreatments of polysaccharides. Flow cytometry results showed that pretreatments with polysaccharides enhanced the phagocytosis of peritoneal macrophages in mice. The increased levels of serum antibody IgG and IgM were observed in the groups pretreated with polysaccharides. Our work demonstrated that the treatment of polysaccharides elicited strong immune activity and a protective effect against cyclophosphamide-induced immunosuppression.
真菌多糖具有多种重要的生物活性，包括抗炎、抗肿瘤和免疫刺激活性。本研究的目的是探讨真菌多糖对环磷酰胺诱导的小鼠免疫抑制的免疫调节作用。分别用香菇多糖、银耳多糖、茯苓多糖或三者混合物对小鼠进行口服预处理。结果表明，多糖预处理显著提高了环磷酰胺诱导的免疫抑制小鼠的胸腺指数。多糖预处理后，环磷酰胺诱导小鼠血清中细胞因子IL-10水平降低。流式细胞术结果表明，多糖预处理增强了小鼠腹腔巨噬细胞的吞噬作用。多糖预处理组血清抗体IgG和IgM水平升高。我们的研究表明，多糖治疗可引发强大的免疫活性，并对环磷酰胺诱导的免疫抑制具有保护作用。
