College of Pharmacy, Kangwon National University, Chuncheon, Gangwon, 24341, Republic of Korea.
R&D Center, Radiant Ltd., Chuncheon, Gangwon, 24398, Republic of Korea.
Phytother Res. 2018 May;32(5):923-932. doi: 10.1002/ptr.6032. Epub 2018 Jan 29.
The therapeutic potentials of the ethanol extract of Artemisia capillaris (ACE) for psoriasis were verified in HaCaT cells (as an immortalized human keratinocyte cell line) and imiquimod (IMQ)-induced psoriasis-like mouse models. In HaCaT cells, IC value of ACE was 37.5 μg/ml after incubating for 72 hr. The antiproliferation activity of ACE in HaCaT cells was further verified by apoptosis assays. The percentage of apoptotic population in ACE-treated group was significantly higher than that of control group (p < .05). The result of cell cycle arrest assay also supported the observed antiproliferation efficacy of ACE in HaCaT cells. In IMQ-induced psoriasis-like mouse models, the Psoriasis Area and Severity Index score of ACE (50 mg/ml; ACE50)-treated group was significantly lower than that of IMQ group on Day 4 (p < .05). After topical application of ACE on psoriasis-like lesion for 4 days, the epidermal thickness of (IMQ + ACE50) group was significantly lower than that of IMQ group (p < .05). The expression levels of Ki-67 and intracellular adhesion molecule-1 in excised skin tissues of (IMQ + ACE50) group were also lower than those of IMQ group. All these findings suggest that ACE can be used as a promising antipsoriatic agent.
艾条乙醇提取物（ACE）治疗银屑病的潜力在 HaCaT 细胞（一种永生化的人角质形成细胞系）和咪喹莫特（IMQ）诱导的银屑病样小鼠模型中得到了验证。在 HaCaT 细胞中，ACE 在孵育 72 小时后，IC 值为 37.5μg/ml。ACE 在 HaCaT 细胞中的增殖抑制活性进一步通过凋亡试验得到验证。ACE 处理组的凋亡细胞比例明显高于对照组（p<0.05）。细胞周期阻滞试验的结果也支持 ACE 在 HaCaT 细胞中观察到的增殖抑制作用。在 IMQ 诱导的银屑病样小鼠模型中，ACE（50mg/ml；ACE50）处理组的银屑病面积和严重程度指数评分在第 4 天明显低于 IMQ 组（p<0.05）。在银屑病样病变处外用 ACE 4 天后，（IMQ+ACE50）组的表皮厚度明显低于 IMQ 组（p<0.05）。（IMQ+ACE50）组切除皮肤组织中的 Ki-67 和细胞间黏附分子-1 的表达水平也低于 IMQ 组。所有这些发现表明 ACE 可作为一种有前途的抗银屑病药物。
