School of Chemical and Biotechnology, SASTRA University, Thirumalaisamudram, Thanjavur, 613401, Tamil Nadu, India.
Department of Advanced Zoology and Biotechnology, Ramakrishna Mission Vivekananda College, Mylapore, Chennai, 600004, India.
Sci Rep. 2018 Feb 2;8(1):2200. doi: 10.1038/s41598-018-20627-3.
Bacterial resistance is a major clinical problem, which is compounded by both a lack of new antibiotics and emergence of multi- and extremely-drug resistant microbes. In this context, non-toxic nanoparticles could play an important role in conferring protection against bacterial infections and in this study we have made an attempt to show the usefulness of jacalin capped platinum nanoparticles in protecting zebrafish against multiple infections with Aeromonas hydrophila. Our results also indicate that use of nanoparticles promotes adaptive immune response against the pathogen, so much so that zebrafish is able to survive repetitive infection even after twenty one days of being treated with jacalin-capped platinum nanoparticles. This is significant given that platinum salt is not antibacterial and jacalin is non-immunogenic. Our study for the first time reveals a novel mechanism of action of nanoparticles, which could form an alternate antibacterial strategy with minimal bacterial resistance.
细菌耐药性是一个主要的临床问题，由于缺乏新的抗生素和多药及极端耐药微生物的出现，这一问题更加复杂。在这种情况下，无毒的纳米粒子可以在防止细菌感染方面发挥重要作用，在这项研究中，我们试图展示麻疯树凝集素包裹的铂纳米粒子在保护斑马鱼免受嗜水气单胞菌多次感染方面的有用性。我们的结果还表明，纳米粒子的使用促进了针对病原体的适应性免疫反应，以至于即使在经过 21 天的麻疯树凝集素包裹的铂纳米粒子治疗后，斑马鱼也能够在重复感染中存活。鉴于铂盐没有抗菌作用，麻疯树凝集素没有免疫原性，这一点意义重大。我们的研究首次揭示了纳米粒子的一种新的作用机制，它可以形成一种具有最小细菌耐药性的替代抗菌策略。
