Wang Qiannan, An Bang, Hou Xingrong, Guo Yunfeng, Luo Hongli, He Chaozu
Hainan Key Laboratory for Sustainable Utilization of Tropical Bioresources and College of Biology, Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, China.
Front Microbiol. 2018 Jan 4;8:2621. doi: 10.3389/fmicb.2017.02621. eCollection 2017.
from is the hemibiotrophic fungi which could cause anthracnose in rubber trees. Dicer like proteins (DCL) were the core enzymes for generation of small RNAs. In the present study, the knocking-out mutants of two dicer like proteins encoding genes of were constructed; and functions of two proteins were investigated. The results showed that DCL play important roles in regulating the growth, conidiation and pathogenicity of ; and there is a functional redundancy between DCL1 and DCL2. Microscopy analysis and DAB staining revealed that loss of penetration ability into the host cells, instead of the decreased growth rate, was the main cause for the impaired pathogenicity of the ΔDcl1ΔDcl2 double mutant. Proteomics analysis suggested that DCL proteins affected the expression of functional proteins to regulating multiple biological processes of . These data lead to a better understanding of the functions of DCL proteins in regulating the development and pathogenesis of .
来自[具体名称未给出]是一种半活体营养型真菌，可导致橡胶树炭疽病。类Dicer蛋白（DCL）是产生小RNA的核心酶。在本研究中，构建了[具体名称未给出]两个类Dicer蛋白编码基因的敲除突变体；并研究了这两种蛋白的功能。结果表明，DCL在调节[具体名称未给出]的生长、分生孢子形成和致病性方面发挥重要作用；并且DCL1和DCL2之间存在功能冗余。显微镜分析和DAB染色表明，ΔDcl1ΔDcl2双突变体致病性受损的主要原因是对宿主细胞的穿透能力丧失，而非生长速率降低。蛋白质组学分析表明，DCL蛋白影响功能蛋白的表达，以调节[具体名称未给出]的多个生物学过程。这些数据有助于更好地理解DCL蛋白在调节[具体名称未给出]的发育和致病性中的功能。
