Bogdanova Katerina, Röderova Magdalena, Kolar Milan, Langova Katerina, Dusek Martin, Jost Petr, Kubelkova Klara, Bostik Pavel, Olsovska Jana
Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, Olomouc, Czech Republic.
Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University, Hnevotinska 3, Olomouc, Czech Republic.
Res Microbiol. 2018 Apr;169(3):127-134. doi: 10.1016/j.resmic.2017.12.005. Epub 2018 Jan 31.
Bacterial biofilms pose a serious medical problem due to their significant resistance to antimicrobials, and staphylococci are recognized as the most frequent cause of biofilm-associated infections. The hop plant (Humulus lupulus L.) contains substances that have been determined to act as anti-infective agents against bacteria, mainly in planktonic form. Therefore, we decided to investigate the antibiofilm properties of H. lupulus L.-derived compounds (humulone, lupulone and xanthohumol) against a selected group of Staphylococcus spp., including methicillin-susceptible and resistant strains. All tested hop compounds were shown to possess antimicrobial properties against all tested staphylococci, both planktonic and biofilm-dwelling, with no significant difference between resistant and susceptible strains. All compounds lowered the number of bacterial cells released from the biofilm, with the strongest effect seen for lupulone, followed by xanthohumol. Moreover, lupulone and xanthohumol were not only able to penetrate the biofilm and reduce the number of bacteria within it, but their higher concentrations (∼60 μg/mL for xanthohumol and ∼125 μg/mL for lupulone) reduced the number of surviving bacterial cells to zero.
由于细菌生物膜对抗菌药物具有显著抗性，因此构成了一个严重的医学问题，葡萄球菌被认为是生物膜相关感染最常见的病因。啤酒花植物（Humulus lupulus L.）含有已被确定可作为抗细菌感染剂的物质，主要针对浮游形式的细菌。因此，我们决定研究来源于啤酒花（Humulus lupulus L.）的化合物（葎草酮、蛇麻酮和黄腐酚）对一组选定的葡萄球菌属细菌的抗生物膜特性，包括甲氧西林敏感和耐药菌株。所有测试的啤酒花化合物均显示对所有测试的葡萄球菌具有抗菌特性，无论是浮游菌还是生物膜中的细菌，耐药菌株和敏感菌株之间没有显著差异。所有化合物都减少了从生物膜中释放的细菌细胞数量，其中蛇麻酮的效果最强，其次是黄腐酚。此外，蛇麻酮和黄腐酚不仅能够穿透生物膜并减少其中的细菌数量，而且它们的较高浓度（黄腐酚约为60μg/mL，蛇麻酮约为125μg/mL）可将存活细菌细胞数量减少至零。
