Piasecki Bartłomiej, Biernasiuk Anna, Ludwiczuk Agnieszka
Department of Pharmacognosy with the Medicinal Plant Garden, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland.
Department of Pharmaceutical Microbiology, Medical University of Lublin, 1 Chodźki Str., 20-093 Lublin, Poland.
Pharmaceuticals (Basel). 2023 Aug 3;16(8):1098. doi: 10.3390/ph16081098.
This study examined the chemical composition and anti-coccal properties of essential oils and methanolic extracts of six different L. varieties from Poland: Iunga, Marynka, Sybilla, Magnum, Tradition and Chinook. The activity of an α-acid-enriched fraction of methanolic extracts was also studied. The chemical composition of essential oils and extracts was determined by gas chromatography-mass spectrometry (GC/MS) and liquid chromatography-mass spectrometry (LC/MS) techniques. The compounds characteristic to extracts include xanthohumol, α-acids, β-acids, and prenylated flavonoids. Essential oil compositions showed a high prevalence of monoterpene hydrocarbon, myrcene and sesquiterpene hydrocarbons, α-humulene and β-caryophyllene. The antimicrobial activity was investigated against eight human cocci pathogenic strains: MRSA (ATCC 43300), MRSA (29213), MSSA (ATCC 29213), (ATCC 12228), (ATCC 29212), VRE (ATCC 51299), (ATCC 19434) and (ATCC 10240). The lowest minimum inhibitory concentrations (MIC) were obtained for extracts and essential oils from Iunga hop samples. Extracts were significantly more active than essential oils. The most susceptible strain to both essential oils and extracts was , whilst the least susceptible was . The antimicrobial activity correlated with a high concentration of xanthohumol of active extracts rather than with the content of α-acids. Xanthohumol showed considerable activity against MRSA with an MIC value of 3.9 µg/mL. The activity of the α-acid-enriched fraction was mediocre compared to the results of all extracts.
尤加（Iunga）、马林卡（Marynka）、西比拉（Sybilla）、玛格南（Magnum）、传统（Tradition）和奇努克（Chinook）的精油及甲醇提取物的化学成分和抗球菌特性。还研究了甲醇提取物中富含α-酸部分的活性。通过气相色谱-质谱联用（GC/MS）和液相色谱-质谱联用（LC/MS）技术测定了精油和提取物的化学成分。提取物的特征性化合物包括黄腐酚、α-酸、β-酸和异戊烯基黄酮。精油成分显示单萜烃、月桂烯以及倍半萜烃、α-葎草烯和β-石竹烯的含量很高。针对八种人类致病性球菌菌株研究了其抗菌活性：耐甲氧西林金黄色葡萄球菌（MRSA，ATCC 43300）、MRSA（29213）、甲氧西林敏感金黄色葡萄球菌（MSSA，ATCC 29213）、（ATCC 12228）、（ATCC 29212）、耐万古霉素肠球菌（VRE，ATCC 51299）、（ATCC 19434）和（ATCC 10240）。尤加啤酒花样品的提取物和精油获得了最低的最低抑菌浓度（MIC）。提取物的活性明显高于精油。对精油和提取物最敏感的菌株是 ，而最不敏感的是 。抗菌活性与活性提取物中高浓度的黄腐酚相关，而非与α-酸的含量相关。黄腐酚对MRSA显示出相当高的活性，MIC值为3.9μg/mL。与所有提取物的结果相比，富含α-酸部分的活性一般。
