Department of Biology, Faculty of Basic Sciences, Hakim Sabzevari University, Sabzevar 96179-76487, Iran.
J Biotechnol. 2018 Mar 20;270:1-11. doi: 10.1016/j.jbiotec.2018.01.021. Epub 2018 Jan 31.
Discovery of new properties and special functionalities at the nanoscale materials caused nanotechnology to become one of the leading parts in all sciences namely biology and medicine. Magnetic iron oxide nanoparticles (MIONPs) are among interesting nanomaterials in biomedical arena, which have attracted the attention of many researchers owing to their extensive capabilities. Due to the simple, cost-effective and environmentally-friendly production processes, biosynthesis is of paramount importance between different methods of nanoparticles production. In the current study, we succeeded to synthesize MIONPs using a newly extracted bacteria supernatant. Produced nanoparticles were characterized using FE-SEM, DLS, VSM, UV-vis, FT-IR and EDS spectroscopy. Analysis showed that the average particle size of very stable spherical MIONPs is about 29.3 nm. The bacteria protein profile obtained by SDS-PAGE analysis indicated induction of different proteins. In vitro cytotoxicity of nanoparticles on the viability of MCF7 and 3T3 cell lines was assessed by MTT assay. The results show that toxicity of the produced nanoparticles (IC > 5 mg/ml and IC > 7.5 mg/ml) follows a concentration dependent manner.
纳米材料新性能和特殊功能的发现促使纳米技术成为所有科学领域（包括生物学和医学）的主要领域之一。磁性氧化铁纳米粒子（MIONPs）是生物医学领域中一种有趣的纳米材料，由于其广泛的应用前景，引起了许多研究人员的关注。由于生产工艺简单、成本低、环保，生物合成在纳米粒子的各种生产方法中显得尤为重要。在本研究中，我们成功地使用新提取的细菌上清液合成了 MIONPs。采用 FE-SEM、DLS、VSM、UV-vis、FT-IR 和 EDS 光谱对所制备的纳米粒子进行了表征。分析表明，非常稳定的球形 MIONPs 的平均粒径约为 29.3nm。通过 SDS-PAGE 分析获得的细菌蛋白图谱表明，不同蛋白被诱导。通过 MTT 法评估了纳米粒子对 MCF7 和 3T3 细胞系活力的体外细胞毒性。结果表明，所制备的纳米粒子（IC>5mg/ml 和 IC>7.5mg/ml）的毒性呈浓度依赖性。
