Department of Medicine, University of Colorado Anschutz Medical Campus, Aurora.
Department of Pediatrics, University of Colorado Anschutz Medical Campus, Aurora.
J Infect Dis. 2018 Mar 13;217(7):1055-1059. doi: 10.1093/infdis/jix653.
We studied the relationship between varicella-zoster virus (VZV) DNAemia and development of VZV-specific immunity after administration of live-attenuated zoster vaccine. VZV-DNAemia, detected by polymerase chain reaction (PCR), and VZV-specific effector (Teff) and memory (Tmem) T cells, was measured in 67 vaccinees. PCR was positive in 56% (9 direct, 28 nested) on day 1 and in 16% (1 direct, 10 nested) on day 14. Teff progressively increased in direct-PCR-positive vaccinees up to day 30, but Tmem did not. Conversely, Tmem, but not Teff, increased in direct-PCR-negative vaccinees on day 7. The kinetics of these immune responses and VZV DNAemia suggested that direct-PCR sample positive represented viremia.
我们研究了水痘带状疱疹病毒 (VZV) DNA 血症与活减毒带状疱疹疫苗接种后 VZV 特异性免疫的发展之间的关系。通过聚合酶链反应 (PCR) 检测 VZV-DNA 血症，并测量 67 名疫苗接种者中的 VZV 特异性效应 (Teff) 和记忆 (Tmem) T 细胞。在第 1 天，PCR 阳性率为 56%（9 个直接检测，28 个巢式检测），在第 14 天为 16%（1 个直接检测，10 个巢式检测）。在直接 PCR 阳性的疫苗接种者中，Teff 逐渐增加，直到第 30 天，但 Tmem 没有增加。相反，在直接 PCR 阴性的疫苗接种者中，Tmem 在第 7 天增加，而 Teff 没有增加。这些免疫反应和 VZV DNA 血症的动力学表明，直接 PCR 样本阳性代表病毒血症。
