Molecular Biology Laboratory, School of Medicine, Universidade Federal do Rio Grande, Rio Grande, Brazil.
Parasitology Laboratory, School of Medicine, Universidade Federal do Rio Grande, Rio Grande, Brazil.
Am J Reprod Immunol. 2018 Apr;79(4):e12824. doi: 10.1111/aji.12824. Epub 2018 Feb 10.
Herpes simplex virus type 2 (HSV-2) infection is one of the most prevalent diseases worldwide and is mainly sexually transmitted. When infecting pregnant women, HSV-2 is able to infect the placenta, can reach the fetus, and may affect the fetal development. We sought to determine the prevalence of HSV-2 infection and reactivation in asymptomatic pregnant women, the correlation between IgG in the maternal circulation and cord blood, and the correlation between circulating IgG, placental, and newborn infection (blood cord).
Serum samples and placental tissues from pregnant women and umbilical cord blood samples from their newborns were collected. Anti-HSV-2 antibodies were identified by ELISA, and HSV-2 DNA was detected by nested PCR.
The seropositivity of IgG in pregnant women was 29.7% and IgM was detected in 1 woman (0.5%). In the umbilical cord of newborns, 33.1% were IgG-positive and IgM was detected in 2 samples (1.5%). A positive correlation between HSV-2 IgG titers in serum from pregnant women and cord blood samples was found (r = .36, P = .001). A difference between the positive and negative placental groups (maternal side) was found in titers of IgG in sera of umbilical cord, which were significantly higher in the positive placental group (P = .004).
We describe for the first time that newborns from mothers with HSV-2 placental infection have higher IgG titers in sera of umbilical cord, suggesting IgGs antibodies can be indicative of placental viral infection in asymptomatic women.
单纯疱疹病毒 2 型（HSV-2）感染是全球最常见的疾病之一，主要通过性传播。当感染孕妇时，HSV-2 能够感染胎盘，到达胎儿，并可能影响胎儿发育。我们旨在确定无症状孕妇中 HSV-2 感染和再激活的流行率、母体循环和脐带血中 IgG 的相关性，以及循环 IgG、胎盘和新生儿感染（脐带血）之间的相关性。
收集孕妇血清样本和胎盘组织以及其新生儿脐带血样本。通过 ELISA 鉴定抗 HSV-2 抗体，通过巢式 PCR 检测 HSV-2 DNA。
孕妇 IgG 血清阳性率为 29.7%，1 例（0.5%）检测到 IgM。新生儿脐带中，33.1% IgG 阳性，2 例（1.5%）检测到 IgM。孕妇血清和脐带血样本中 HSV-2 IgG 滴度呈正相关（r=.36，P=.001）。在胎盘阳性组和阴性组之间（母体侧）发现血清 IgG 滴度存在差异，胎盘阳性组的 IgG 滴度明显更高（P=.004）。
我们首次描述了 HSV-2 胎盘感染母亲的新生儿脐带血清中 IgG 滴度较高，表明 IgG 抗体可提示无症状妇女的胎盘病毒感染。
