Cheng Lin, Shi Liang, Wu Jing, Zhou Xujie, Li Xiaoxia, Sun Xi, Zhu Lei, Xia Tian-Song, Ding Qiang
Jiangsu Breast Disease Center, The First Affiliated Hospital with Nanjing Medical University, Nanjing, Jiangsu 210029, P.R. China.
Department of Breast Surgery, Changzhou No. 2 People's Hospital, Affiliated to Nanjing Medical University, Changzhou, Jiangsu 213000, P.R. China.
Oncol Lett. 2018 Feb;15(2):1737-1743. doi: 10.3892/ol.2017.7494. Epub 2017 Nov 27.
Natural plants offer a treasure trove of resources for anticancer drug development. are widely used in Traditional Chinese Medicine. However, studies on the active substances in are scarce. In the present study, four monomer compounds were successfully extracted from this species and their inhibitory effects on the growth of breast cancer cells were investigated using bioactivity tests. Among them, hederagenin saponin (CHS) belongs to the class of triterpenoid saponins. CHS showed cytotoxic effects on breast cancer cells in a dose- and time-dependent manner. The compound also induced apoptosis in breast cancer cells in a time-dependent manner. Further investigation into the underlying mechanisms of apoptosis induction in breast cancer cells showed that the compound significantly reduced mitochondrial Apaf-1 and cytochrome proteins in breast cancer cells. In addition, it upregulated the activities of caspase-3 and -9. In conclusion, CHS induced apoptosis in breast cancer cells through regulation of the mitochondrial apoptosis pathway. The results suggest that the hederagenin saponin extracted from offers great potential as a novel anti-breast cancer drug.
天然植物为抗癌药物开发提供了丰富的资源宝库。它们在传统中药中被广泛使用。然而，对其活性物质的研究却很匮乏。在本研究中，成功从该物种中提取了四种单体化合物，并通过生物活性测试研究了它们对乳腺癌细胞生长的抑制作用。其中，常春藤皂苷元皂苷（CHS）属于三萜皂苷类。CHS对乳腺癌细胞具有剂量和时间依赖性的细胞毒性作用。该化合物还以时间依赖性方式诱导乳腺癌细胞凋亡。对乳腺癌细胞凋亡诱导的潜在机制的进一步研究表明，该化合物显著降低了乳腺癌细胞中线粒体Apaf-1和细胞色素蛋白的含量。此外，它上调了caspase-3和-9的活性。总之，CHS通过调节线粒体凋亡途径诱导乳腺癌细胞凋亡。结果表明，从该植物中提取的常春藤皂苷元皂苷作为一种新型抗乳腺癌药物具有巨大潜力。
