Department of Breast Surgery, First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, 210029, China.
J Ethnopharmacol. 2009 Dec 10;126(3):382-5. doi: 10.1016/j.jep.2009.09.028. Epub 2009 Sep 23.
The roots and rhizomes of Clematis are commonly used as an analgesic, abirritative, antibacterial, antiphlogistic, anticancer and diuretic agent. The Naxi people traditionally used Clematis ganpiniana's (Lévl. Et Vant.) as a diuretic agent, an anti-inflammatory and anticancer remedy.
To investigate the cytotoxic and antibacterial components from Clematis ganpiniana.
The aboveground part of Clematis ganpiniana was isolated by chromatographic techniques. Structures of isolated compounds were identified by spectroscopic methods and comparison with published data. Their cytotoxic, apoptosis and antibacterial activities of purified components were also performed.
By bioassay-guided fractionation techniques and chemical characterization, four triterpene glycosides were isolated and their cytotoxicity against cancer cells and antibacterial activity were tested. They showed significant inhibitory activities against MCF-7, MDA-MB-231 with IC50 value of 0.7-16.5 microg/ml, and significant apoptosis for MCF-7 and MDA-MB-231. Moreover, compound 4 showed weak wide-spectrum antibacterial activity.
These results provide promising baseline information for the potential use of Clematis ganpiniana as well as some of the isolated compounds in the treatment of cancer and infectious disease.
铁线莲的根和根茎通常被用作镇痛药、镇咳药、抗菌药、消炎药、抗癌药和利尿剂。纳西族传统上将 Clematis ganpiniana (Lévl. Et Vant.) 用作利尿剂、消炎药和抗癌药物。
从 Clematis ganpiniana 中分离具有细胞毒性和抗菌作用的成分。
采用色谱技术从Clematis ganpiniana 的地上部分进行分离。通过光谱方法和与已发表数据的比较确定分离化合物的结构。还对纯化成分的细胞毒性、细胞凋亡和抗菌活性进行了测试。
通过基于生物测定的分离技术和化学表征，分离出四种三萜糖苷，并测试了它们对癌细胞的细胞毒性和抗菌活性。它们对 MCF-7、MDA-MB-231 的抑制活性较强，IC50 值为 0.7-16.5 μg/ml，对 MCF-7 和 MDA-MB-231 具有明显的凋亡作用。此外，化合物 4 还表现出较弱的广谱抗菌活性。
这些结果为铁线莲的潜在用途以及一些分离化合物在癌症和传染病治疗中的应用提供了有希望的基础信息。
