Kukula-Koch Wirginia
Chair and Department of Pharmacognosy with Medicinal Plants Unit, 1 Chodzki St., 20-093 Lublin, Poland.
J Anal Methods Chem. 2017;2017:8384107. doi: 10.1155/2017/8384107. Epub 2017 Dec 25.
Twenty-five methanol extracts obtained from various representatives of Papaveraceae and Berberidaceae botanical families (genera: , , , , , and ) were screened for their alkaloid content in an optimized method suitable for the LC-ESI-Q-TOF-MS analysis. Twelve pharmacologically important isoquinoline alkaloids from four groups, aporphines, benzylisoquinolines, protoberberines, and benzophenanthridines, present in these traditionally used plant species were quantitatively determined in each studied sample, providing their alkaloid profile. A Zorbax Stable Bond RP-18 column and a mobile phase composed of 0.1% formic acid and 0.1% formic acid in acetonitrile (v/v) were used at the flow rate of 0.2 mL/min. A profound study on the optimization of MS response to four groups of isoquinoline alkaloids (validation of capillary voltage, gas flows, nebulizer pressure, skimmer, and fragmentor voltages), repeatability of results, and stability and linearity of measurements were described, showing, among others, 3000 V of capillary voltage, 350°C of gas temperature, 12 L/min of gas flows, nebulizer pressure of 35 psig, 65 V for skimmer voltage, and 30 V for collision energy as the most advantageous operation parameters.
对从罂粟科和小檗科植物家族的各种代表植物（属： 、 、 、 、 、 ）中获得的25种甲醇提取物进行筛选，采用适合液相色谱-电喷雾电离-四极杆-飞行时间质谱（LC-ESI-Q-TOF-MS）分析的优化方法检测其生物碱含量。对这些传统药用植物物种中存在的来自阿朴啡碱、苄基异喹啉、原小檗碱和苯并菲啶四类的12种具有重要药理作用的异喹啉生物碱在每个研究样品中进行了定量测定，给出了它们的生物碱图谱。使用Zorbax Stable Bond RP-18柱和由0.1%甲酸和乙腈中0.1%甲酸（体积比）组成的流动相，流速为0.2 mL/min。描述了对四类异喹啉生物碱的质谱响应优化（毛细管电压、气体流速、雾化器压力、分离器和碎裂器电压的验证）、结果的重复性以及测量的稳定性和线性的深入研究，结果表明，除其他外，3000 V的毛细管电压、350°C的气体温度、12 L/min的气体流速、35 psig的雾化器压力、65 V的分离器电压和30 V的碰撞能量是最有利的操作参数。
