From the Department of Biological Sciences and the Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180 and
From the Department of Biological Sciences and the Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, New York 12180 and.
J Biol Chem. 2018 Mar 23;293(12):4510-4518. doi: 10.1074/jbc.R117.001324. Epub 2018 Feb 14.
Kinesin-2s are major transporters of cellular cargoes. This subfamily contains both homodimeric kinesins whose catalytic domains result from the same gene product and heterodimeric kinesins with motor domains derived from two different gene products. In this Minireview, we focus on the progress to define the biochemical and biophysical properties of the kinesin-2 family members. Our understanding of their mechanochemical capabilities has been advanced by the ability to identify the kinesin-2 genes in multiple species, expression and purification of these motors for single-molecule and ensemble assays, and development of new technologies enabling quantitative measurements of kinesin activity with greater sensitivity.
驱动蛋白-2 是细胞货物的主要转运蛋白。这个亚家族包含两种同源二聚体驱动蛋白，它们的催化结构域来自同一个基因产物，以及异源二聚体驱动蛋白，它们的马达结构域来自两个不同的基因产物。在这篇综述中，我们专注于定义驱动蛋白-2 家族成员的生化和生物物理特性的进展。通过能够在多种物种中识别驱动蛋白-2 基因、表达和纯化这些用于单分子和整体测定的马达，以及开发新的技术来提高驱动蛋白活性的定量测量的灵敏度，我们对它们的机械化学能力的理解得到了推进。
