Gutt Beatrice, Ren Qun, Hauser-Gerspach Irmgard, Kardas Piotr, Stübinger Stefan, Astasov-Frauenhoffer Monika, Waltimo Tuomas
Laboratory for Biointerfaces, Swiss Federal Laboratories for Materials Science and Technology, St. Gallen, Switzerland.
Department of Preventive Dentistry and Oral Microbiology, University Center for Dental Medicine, University of Basel, Basel, Switzerland.
Front Microbiol. 2018 Jan 31;9:107. doi: 10.3389/fmicb.2018.00107. eCollection 2018.
Periodontitis is a very common health problem caused by formation of pathogenic bacterial biofilm that triggers inflammation resulting in either reversible gingivitis or irreversible periodontal hard and soft tissue damages, leading to loss of teeth when left untreated. Commensal bacteria play an important role in oral health in many aspects. Mainly by colonizing oral tissues, they (i) contribute to maturation of immune response, and (ii) foreclose attachment of pathobiont and, therefore, prevent from infection. The main goal of the study was to investigate if blocking of receptors on a commensal biofilm can prevent or reduce the attachment of pathogenic strains. To do so, biofilm produced by commensal was treated with whole cell lysate of pathobionts or , followed by incubation with respective strain(s). The study revealed significant reduction in pathobiont adhesion to lysate-treated commensal biofilm. Therefore, adhesion of pathobionts onto the lysate-blocked biofilm was hindered; however, not completely eliminated supporting the idea that such approach in the oral cavity would benefit the production of a well-balanced and healthy bioactive interface.
牙周炎是一种非常常见的健康问题，由致病性细菌生物膜的形成引起，这种生物膜会引发炎症，导致可逆性牙龈炎或不可逆的牙周软硬组织损伤，若不治疗会导致牙齿脱落。共生细菌在口腔健康的许多方面发挥着重要作用。它们主要通过在口腔组织中定殖，（i）促进免疫反应的成熟，以及（ii）阻止致病共生菌的附着，从而预防感染。该研究的主要目的是调查阻断共生生物膜上的受体是否可以预防或减少致病菌株的附着。为此，用致病共生菌或的全细胞裂解物处理共生菌产生的生物膜，然后与相应的菌株一起孵育。研究表明，致病共生菌对经裂解物处理的共生生物膜的粘附显著减少。因此，致病共生菌在裂解物阻断的生物膜上的粘附受到阻碍；然而，并未完全消除，这支持了这样一种观点，即在口腔中采用这种方法将有利于产生平衡且健康的生物活性界面。
