Hanel Alyssa N, Herzog Hannah M, James Michelle G, Cuadra Giancarlo A
Department of Biology, Muhlenberg College, 2400 W. Chew Street, Allentown, PA 18104, USA.
College of Dental Medicine, Columbia University, 622 W 168th St, New York, NY 10032, USA.
Dent J (Basel). 2020 May 2;8(2):39. doi: 10.3390/dj8020039.
The objective of this study was to determine if the interaction between common oral commensal bacteria and oral epithelial cells would provide protective effects against the invasion of periodontopathogen . Oral epithelial OKF6/Tert cells were used in co-cultures with , , , and . The viability of OKF6/Tert cells following a bacterial challenge was evaluated by trypan blue exclusion. The adherence of commensal species was determined by CFU counts. invasion in OKF6/Tert cells was assessed before and after exposure to commensal species according to CFU counts. Viability assays show that only and display low toxicity toward OKF6/Tert cells. Both commensals adhere to OKF6/Tert cells at an average ratio of 1 CFU to 10 cells. invasion into host cells is significantly reduced by 25% or 60% after exposure to or , respectively. The results suggest that these commensal species bind to host cells and diminish invasion. This is important in the context of periodontal disease since primarily acts on the host by invading it. Therefore, efforts to decrease invasion will eventually lead to future therapies harnessing the mechanisms employed by oral commensal bacteria.
本研究的目的是确定常见口腔共生菌与口腔上皮细胞之间的相互作用是否会对牙周病原体的入侵产生保护作用。口腔上皮OKF6/Tert细胞与[具体细菌名称1]、[具体细菌名称2]、[具体细菌名称3]和[具体细菌名称4]进行共培养。通过台盼蓝排斥法评估细菌攻击后OKF6/Tert细胞的活力。通过菌落形成单位（CFU）计数确定共生菌的黏附情况。根据CFU计数评估在暴露于共生菌之前和之后OKF6/Tert细胞中[牙周病原体名称]的入侵情况。活力测定表明，只有[具体细菌名称1]和[具体细菌名称2]对OKF6/Tert细胞显示出低毒性。两种共生菌均以平均1个CFU对应10个细胞的比例黏附于OKF6/Tert细胞。在暴露于[具体细菌名称1]或[具体细菌名称2]后，[牙周病原体名称]对宿主细胞的入侵分别显著降低了25%或60%。结果表明，这些共生菌与宿主细胞结合并减少了[牙周病原体名称]的入侵。在牙周疾病的背景下，这一点很重要，因为[牙周病原体名称]主要通过侵入宿主来发挥作用。因此，减少入侵的努力最终将导致未来利用口腔共生菌所采用机制的治疗方法。
