Bioengineering Laboratory, Department of Textile Technology, Indian Institute of Technology Delhi, New Delhi 110016, India.
Department of Pathology, Sikkim Manipal Institute of Medical Sciences, Sikkim Manipal University, Gangtok 737102, India.
Int J Biol Macromol. 2018 Jul 1;113:227-239. doi: 10.1016/j.ijbiomac.2018.02.097. Epub 2018 Feb 15.
Dextran based bionanocomposite membranes encapsulating clove oil (CO) and sandalwood oil (SO) that are capable of preventing infection due to their inherent virtue of antibacterial activity and modifying the wound healing cascade for accelerated scar free healing, were developed. A facile solvent casting technique was used to fabricate dextran/nanosoy/glycerol/chitosan (DNG/Ch) nanocomposite membranes followed by subsequent addition of CO and SO to obtain DNG/Ch/CO and DNG/Ch/SO herbal nanodressings. Dressings exhibited >98% antibacterial activity against both Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) at extremely low loadings of 5% and 10% for CO and SO, respectively. This encapsulation strategy allowed controlled diffusion of EO over a period of 72h which was measured in terms of drug efficacy using bacterial reduction count test and serial plate transfer disk diffusion test (SPTDDT). Swelling behavior and mechanical properties were also examined. Bacterial adherence study was performed to demonstrate the efficiency of dressings for arresting microbial invasion. In vivo wound healing studies were conducted using male swiss albino mice of BALB/c strain and DNG/Ch/CO dressings exhibited complete healing within 14days with remarkable efficacy in scar prevention. Histological analysis revealed that CO and SO treatment led to deposition of ordered collagen along with fibroblast migration.
开发了一种基于葡聚糖的生物纳米复合膜，其中封装了丁香油（CO）和檀香油（SO），由于其固有的抗菌活性，能够防止感染，并修饰伤口愈合级联反应，实现无疤痕的快速愈合。采用简单的溶剂铸造技术制备了葡聚糖/纳米大豆/甘油/壳聚糖（DNG/Ch）纳米复合材料膜，然后再加入 CO 和 SO，得到 DNG/Ch/CO 和 DNG/Ch/SO 草药纳米敷料。敷料对金黄色葡萄球菌（S. aureus）和大肠杆菌（E. coli）均表现出>98%的抗菌活性，CO 和 SO 的负载量分别低至 5%和 10%。这种封装策略允许 EO 在 72 小时内进行控制释放，通过细菌减少计数测试和连续平板转移圆盘扩散测试（SPTDDT）来衡量药物功效。还检查了溶胀行为和机械性能。进行了细菌粘附研究，以证明敷料在阻止微生物入侵方面的效率。使用雄性瑞士白化病小鼠（BALB/c 品系）进行体内伤口愈合研究，结果表明 DNG/Ch/CO 敷料在 14 天内完全愈合，在预防疤痕方面具有显著效果。组织学分析表明，CO 和 SO 处理导致有序胶原沉积和成纤维细胞迁移。
