可注射硫酸葡聚糖钠纳米粒的制剂与评价：一种有效的抗菌剂。

Formulation and evaluation of injectable dextran sulfate sodium nanoparticles as a potent antibacterial agent.

机构信息

Department of Pharmaceutics, College of Pharmacy, Jazan University, P.O. Box 114, Jazan, Postal Code 45142, Saudi Arabia.

Department of Pharmaceutics, College of Pharmacy, Umm Al-Qura University, Makkah, 24381, Saudi Arabia.

出版信息

Sci Rep. 2021 May 10;11(1):9914. doi: 10.1038/s41598-021-89330-0.

DOI:10.1038/s41598-021-89330-0

PMID:33972626

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8110975/

Abstract

The purpose of this study was to develop a novel nano antibacterial formulation of dextran sulfate sodium polymer. The dextran sulfate sodium (DSS) nanoparticles were formulated with gelation technique. The nanoparticles exhibited significant physicochemical and effective antibacterial properties, with zeta potential of - 35.2 mV, particle size of 69.3 z d nm, polydispersity index of 0.6, and percentage polydispersity of 77.8. The DSS nanoparticles were stable up to 102 °C. Differential scanning calorimetry revealed an endothermic peak at 165.77 °C in 12.46 min, while XRD analysis at 2θ depicted various peaks at 21.56°, 33.37°, 38.73°, 47.17°, 52.96°, and 58.42°, indicating discrete nanoparticle formation. Antibacterial studies showed that the DSS nanoparticles were effective against Gram-positive and Gram-negative bacteria. The minimum inhibitory concentrations of DSS nanoparticles for Bacillus subtilis (B. subtilis), Staphylococcus aureus (S. aureus), Streptococcus pyogenes (S. pyogenes), Escherichia coli (E. coli), Pseudomonas aeruginosa (P. aeruginosa), Klebsiella pneumoniae (K. pneumoniae) and Proteus vulgaris (P. vulgaris) were 150, 200, 250, 150, 200, 250, 250 µg/mL, respectively. The antibacterial effects of DSS nanoparticles were in the order E. coli (26 ± 1.2 mm) at 150 µg/mL > S. pyogenes (24.6 ± 0.8 mm) at 250 µg/mL > B. subtilis (23.5 ± 2 mm) at 150 µg/mL > K. pneumoniae (22 ± 2 mm) at 250 µg/mL > P. aeruginosa (21.8 ± 1 mm) at 200 µg/mL > S. aureus (20.8 ± 1 mm) at 200 µg/mL > P. vulgaris (20.5 ± 0.9 mm) at 250 µg/mL. These results demonstrate the antibacterial potency of DSS injectable nanoparticles.

摘要

本研究旨在开发一种新型的葡聚糖硫酸钠盐聚合物纳米抗菌制剂。葡聚糖硫酸钠（DSS）纳米粒子采用凝胶技术进行制剂。纳米粒子表现出显著的物理化学和有效的抗菌性能，其 ζ 电位为-35.2 mV，粒径为 69.3 z 径 nm，多分散指数为 0.6，多分散性百分比为 77.8。DSS 纳米粒子在高达 102°C 时稳定。差示扫描量热法在 12.46 分钟时显示出 165.77°C 的吸热峰，而 X 射线衍射分析在 2θ 处显示出各种峰在 21.56°、33.37°、38.73°、47.17°、52.96°和 58.42°，表明离散的纳米粒子形成。抗菌研究表明，DSS 纳米粒子对革兰氏阳性和革兰氏阴性细菌均有效。DSS 纳米粒子对枯草芽孢杆菌（B. subtilis）、金黄色葡萄球菌（S. aureus）、化脓性链球菌（S. pyogenes）、大肠杆菌（E. coli）、铜绿假单胞菌（P. aeruginosa）、肺炎克雷伯菌（K. pneumoniae）和普通变形杆菌（P. vulgaris）的最小抑菌浓度分别为 150、200、250、150、200、250、250μg/mL。DSS 纳米粒子的抗菌效果依次为大肠杆菌（E. coli）（150μg/mL 时为 26±1.2mm）>化脓性链球菌（S. pyogenes）（250μg/mL 时为 24.6±0.8mm）>枯草芽孢杆菌（B. subtilis）（150μg/mL 时为 23.5±2mm）>肺炎克雷伯菌（K. pneumoniae）（250μg/mL 时为 22±2mm）>铜绿假单胞菌（P. aeruginosa）（200μg/mL 时为 21.8±1mm）>金黄色葡萄球菌（S. aureus）（200μg/mL 时为 20.8±1mm）>普通变形杆菌（P. vulgaris）（250μg/mL 时为 20.5±0.9mm）。这些结果表明 DSS 可注射纳米粒子具有抗菌效力。