Zhang Xiaorong, Liu Xiupian, Lai Qiliang, Du Yaping, Sun Fengqin, Shao Zongze
State Key Laboratory Breeding Base of Marine Genetic Resources; Key Laboratory of Marine Biogenetic Resources, Third Institute of Oceanography, SOA; Fujian Collaborative Innovation Center for Exploitation and Utilization of Marine Biological Resources; Key Laboratory of Marine Genetic Resources of Fujian Province, Xiamen 361005, PR China.
Int J Syst Evol Microbiol. 2018 Mar;68(3):881-885. doi: 10.1099/ijsem.0.002602.
A taxonomic study was carried out on strain 3PC125-7, which was isolated from the deep sea water of the Indian Ocean. The bacterium was rod-shaped, non-flagellated, Gram-stain-negative, oxidase- and catalase-positive and strictly aerobic. Optimal growth was observed at 25-37 °C, at pH 7 and in 1-3 % (w/v) NaCl. On the basis of the results of 16S rRNA gene sequence analysis, strain 3PC125-7 represents a member of the genus with the highest sequence similarity to CL-SS4 (96.7 %), followed by H19-56 (96.7 %) and nine other species of the genus (93.5-95.8 %). The principal fatty acids of 3PC125-7 were iso-C, iso-C 3-OH and iso-CG and the sole respiratory quinone was menaquinone-6. The polar lipids comprise phosphatidylethanolamine, six unidentified phospholipids and three unknown lipids. The genomic DNA G+C content of 3PC125-7 was 41.8 mol%. Based on the phylogenetic, phenotypic and chemotaxonomic data obtained in this study, strain 3PC125-7 is considered to represent a novel species in the genus for which the name sp. nov. is proposed, with the type strain 3PC125-7 (=MCCC 1A03198=KCTC 52318).
对从印度洋深海海水中分离得到的3PC125 - 7菌株进行了分类学研究。该细菌呈杆状，无鞭毛，革兰氏染色阴性，氧化酶和过氧化氢酶阳性，严格需氧。在25 - 37°C、pH值为7以及1 - 3%（w/v）NaCl条件下观察到最佳生长。基于16S rRNA基因序列分析结果，3PC125 - 7菌株是该属的一个成员，与CL - SS4的序列相似性最高（96.7%），其次是H19 - 56（96.7%）以及该属的其他九个物种（93.5 - 95.8%）。3PC125 - 7的主要脂肪酸为异-C、异-C 3 - OH和异-CG，唯一的呼吸醌为甲基萘醌-6。极性脂质包括磷脂酰乙醇胺、六种未鉴定的磷脂和三种未知脂质。3PC125 - 7的基因组DNA G + C含量为41.8 mol%。基于本研究获得的系统发育、表型和化学分类学数据，3PC125 - 7菌株被认为代表该属中的一个新物种，为此提出新物种名称sp. nov.，模式菌株为3PC125 - 7（=MCCC 1A03198 = KCTC 52318）。
