Department of Energy and Environment, National Institute of Technology, Tiruchirappalli, India.
Department of Biotechnology, Vels University, Chennai, India.
Int Wound J. 2018 Jun;15(3):350-362. doi: 10.1111/iwj.12872. Epub 2018 Feb 26.
A peptide might be an exciting biomaterial or template for the development of novel wound-healing agents. In this report, it was isolated from the terrestrial snail Cryptozona bistrialis by enzymatic digestion and was evaluated for its in vitro wound-healing activity in NIH/3T3 mouse fibroblasts cell line and in vivo wound-healing activity in normal and diabetic-induced Wistar albino rats. The C. bistrialis protein was digested by the papain enzyme, and 21.79 kDa peptide (Cb-peptide) was purified by reversed-phase high-performance liquid chromatography and identified by MALDI (matrix-assisted laser desorption/ionization)-TOF analysis. The isolated Cb-peptide was characterised by various analytical methods. The peptide demonstrated a capacity to prevent the development of pathogenic bacterial and fungal cultures and proved that it promotes significant wound-healing activity in the wound scratch assay method by rapid cell migration and closure of wound. Isolated Cb-peptide was lyophilised and formulated to ointment and analysed for in vivo wound-healing activity in normal and diabetic (alloxan monohydrate)-induced Wistar albino rats. Cb-peptide ointment-treated groups showed a greater degree of wound healing and early and complete period of epithelialisation in normal and diabetic-induced Wistar albino rats. Cb-peptide ointment-treated groups showed significant excision and incision wound-healing activity. A conclusion was reached that the peptide isolated from C. bistrialis showed greater wound-healing activity compared with vehicle control and standard control.
一种肽可能是一种令人兴奋的生物材料或模板，可用于开发新型伤口愈合剂。在本报告中，该肽从陆生蜗牛 Cryptozona bistrialis 通过酶解分离得到，并在 NIH/3T3 小鼠成纤维细胞系中进行了体外伤口愈合活性评估，在正常和糖尿病诱导的 Wistar 白化大鼠中进行了体内伤口愈合活性评估。用木瓜蛋白酶酶解 C. bistrialis 蛋白，通过反相高效液相色谱法纯化 21.79 kDa 肽（Cb-肽），并通过 MALDI（基质辅助激光解吸/电离）-TOF 分析鉴定。分离的 Cb-肽通过各种分析方法进行了表征。该肽具有防止致病细菌和真菌培养物发展的能力，并通过快速细胞迁移和伤口闭合，证明其在伤口划痕测定法中具有显著的伤口愈合活性。分离的 Cb-肽被冻干并配制成软膏，并在正常和糖尿病（一水合阿脲）诱导的 Wistar 白化大鼠中分析其体内伤口愈合活性。用 Cb-肽软膏处理的组在正常和糖尿病诱导的 Wistar 白化大鼠中显示出更大程度的伤口愈合和早期及完整的上皮化。Cb-肽软膏处理的组显示出明显的切除和切口愈合活性。得出的结论是，与载体对照和标准对照相比，从 C. bistrialis 中分离的肽显示出更大的伤口愈合活性。
