Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Medical Microbiology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
J Photochem Photobiol B. 2018 Apr;181:23-30. doi: 10.1016/j.jphotobiol.2018.02.023. Epub 2018 Feb 21.
The purpose of the present scientific study was to analyze the effects of combined pulsed wave Photobiomodulation (PW PBM) and Curcumin on the microbial flora; in addition, the tensiometrical wounds properties for type one diabetes mellitus (TIDM) in an experimental animal model. TIDM induction was performed in thirty rats. In the entire animals, one full-thickness excision was implemented on their backs. Randomly, the divisions of rats into 5 groups took place. The primary group was considered as the control group and did not receive any treatment. The secondary group (placebo) received sesame oil by gastric gavage. The third group received PWPBM (890 nm, 80 Hz, 0.2 J/cm). The fourth group received curcumin (40 mg/kg, which was dissolved in sesame oil) by gastric gavage. Eventually, the fifth group received PW PBM + curcumin. Precisely, on day 7, microbiological examinations, and on the 15th day microbiological and tensiometrical examinations were conducted. The data were analyzed by statistical tests. PW PBM, significantly exacerbated tensiometrical properties of the TIDM repairing wound. PW PBM, curcumin, and PWPBM + curcumin significantly decreased colony forming units compared to the control and the placebo groups indeed. It was remarkably attained that PW PBM significantly accelerated the process of wound healing in the STZ-induced TIDM. The PW PBM was statistically more compelling compared to the curcumin and PWPBM + curcumin. PW PBM, curcumin, and PWPBM + curcumin significantly decreased colony forming units compared to the control and placebo groups.
本科学研究的目的是分析联合脉冲光光生物调节（PWPBM）和姜黄素对微生物群落的影响；此外，还研究了 PWPBM 和姜黄素对实验动物 1 型糖尿病（TIDM）的创面张力特性的影响。在 30 只大鼠中进行 TIDM 诱导。在所有动物中，它们的背部都进行了全厚切除。随机将大鼠分为 5 组。第一组被认为是对照组，未接受任何治疗。第二组（安慰剂）通过胃管给予芝麻油。第三组接受 PWPBM（890nm，80Hz，0.2J/cm）。第四组通过胃管给予姜黄素（40mg/kg，溶于芝麻油）。最后，第五组接受 PWPBM+姜黄素。第 7 天进行微生物学检查，第 15 天进行微生物学和张力学检查。数据通过统计检验进行分析。PWPBM 显著加重了 TIDM 修复创面的张力特性。与对照组和安慰剂组相比，PWPBM、姜黄素和 PWPBM+姜黄素组的菌落形成单位明显减少。令人惊讶的是，PWPBM 显著加速了 STZ 诱导的 TIDM 创面愈合过程。与姜黄素和 PWPBM+姜黄素相比，PWPBM 在统计学上更有说服力。与对照组和安慰剂组相比，PWPBM、姜黄素和 PWPBM+姜黄素组的菌落形成单位明显减少。
