利用 UPLC-QTOF-MS/MS 鉴定薏苡仁麸皮中的游离酚类物质，并研究酚酸类物质对黄嘌呤氧化酶的抑制机制。
Identification of the free phenolic profile of Adlay bran by UPLC-QTOF-MS/MS and inhibitory mechanisms of phenolic acids against xanthine oxidase.
机构信息
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510640, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China; Beijing Advanced Innovation Center for Food Nutrition and Human Health, Beijing Technology & Business University, Beijing 100048, China; Guangdong Food Green Processing and Nutrition Regulation Technologies Research Center, Guangzhou 510640, China.
出版信息
Food Chem. 2018 Jul 1;253:108-118. doi: 10.1016/j.foodchem.2018.01.139.
Adlay bran free phenolic extract has been previously demonstrated to possess potent xanthine oxidase (XOD) inhibitory activity. The aims of this study were to characterize the free phenolic profile of adlay bran and investigate the structure-activity relationship, underlying mechanism and interaction of phenolic acids as XOD inhibitors. A total of twenty phenolics including ten phenolic acids, two coumarins, two phenolic aldedhyes and six flavonoids were identified in a phenolic compound-guided separation by UPLC-QTOF-MS/MS. Adlay bran free phenolic extract possessed strong XOD inhibitory activity related to hydroxycinnamic acids with methoxyl groups. The hydrogen bonding and hydrophobic interactions were the main forces in the binding of adlay phenolics to XOD. Sinapic acid, identified in adlay bran for the first time, possessed strong XOD inhibitory activity in a mixed non-competitive manner, and synergistic effects with other adlay phenolic acids at low concentrations, and would be a promising agent for preventing and treating hyperuricemia.
薏苡麸游离酚提取物先前已被证明具有很强的黄嘌呤氧化酶（XOD）抑制活性。本研究旨在对薏苡麸游离酚的组成进行鉴定，并对其作为 XOD 抑制剂的构效关系、作用机制和相互作用进行研究。通过 UPLC-QTOF-MS/MS 引导的酚类化合物分离，共鉴定出 20 种酚类化合物，包括 10 种酚酸、2 种香豆素、2 种酚醛和 6 种类黄酮。薏苡麸游离酚提取物具有很强的 XOD 抑制活性，与含甲氧基的羟基肉桂酸有关。氢键和疏水相互作用是薏苡酚与 XOD 结合的主要作用力。阿魏酸是首次在薏苡麸中发现的，它以混合非竞争性方式具有很强的 XOD 抑制活性，并在低浓度下与其他薏苡酚类酸具有协同作用，有望成为预防和治疗高尿酸血症的一种有前途的药物。
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