Araújo Bruna Fuga, Ferreira Melina Lorraine, Campos Paola Amaral de, Royer Sabrina, Gonçalves Iara Rossi, da Fonseca Batistão Deivid William, Fernandes Miriam Rodriguez, Cerdeira Louise Teixeira, Brito Cristiane Silveira de, Lincopan Nilton, Gontijo-Filho Paulo Pinto, Ribas Rosineide Marques
Institute of Biomedical Sciences (ICBIM), Laboratory of Molecular Microbiology, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil.
School of Medicine, Federal University of Uberlandia, Uberlandia, Minas Gerais, Brazil.
J Med Microbiol. 2018 Apr;67(4):523-528. doi: 10.1099/jmm.0.000711. Epub 2018 Mar 6.
In this study, we describe the frequency of virulence genes in Klebsiella pneumoniae carbapenemase-2-producing Klebsiella pneumoniae (KPC-KP), including hypervirulent (hv) and hypermucoviscous (hm) strains by whole-genome sequencing. We also evaluate the capacity for biofilm formation by using phenotypic techniques. The occurrence of several virulence genes (fimABCDEFGHIK, mrkABCDFHJ, ecpA, wabG, entB, ugE, irp1, irp2, traT, iutA and ureADE) and a high frequency of hvhmKPC-KP isolates was found. Most hospital-associated lineages of KPC-KP belong to the international clonal group 258 (CG258). Biofilm formation was a constant feature among 90.9 % of KPC-KP strains. This report suggests a close relationship between ST437 and weak biofilm production, given that all weakly biofilm-producing strains belonged to this sequence type. This also supports the dissemination of KPC-KP containing numerous virulence determinants belonging to the biofilm-producing CG258 type in Brazil, including hv and hm strains. These factors allow this pathogen to cause infections, leading to its rapid expansion and persistence in hospital settings.
在本研究中，我们通过全基因组测序描述了产肺炎克雷伯菌碳青霉烯酶-2的肺炎克雷伯菌（KPC-KP）中致病基因的频率，包括高毒力（hv）和高黏液性（hm）菌株。我们还使用表型技术评估了生物膜形成能力。发现了几种致病基因（fimABCDEFGHIK、mrkABCDFHJ、ecpA、wabG、entB、ugE、irp1、irp2、traT、iutA和ureADE）的存在以及高频率的hvhmKPC-KP分离株。大多数与医院相关的KPC-KP谱系属于国际克隆群258（CG258）。生物膜形成是90.9%的KPC-KP菌株的一个恒定特征。鉴于所有弱生物膜产生菌株均属于该序列类型，本报告表明ST437与弱生物膜产生之间存在密切关系。这也支持了在巴西含有众多属于生物膜产生型CG258的致病决定因素的KPC-KP的传播，包括hv和hm菌株。这些因素使这种病原体能够引起感染，导致其在医院环境中迅速传播和持续存在。
