Musumeci Teresa, Leonardi Antonio, Bonaccorso Angela, Pignatello Rosario, Puglisi Giovanni
Department of Drug Sciences, Research Centre on Ocular Nanotechnology (NANO-i), University of Catania, Catania, Italy.
Pharm Nanotechnol. 2018;6(1):48-60. doi: 10.2174/2211738506666180306160921.
Purification is a key step for different types of approaches, ranging from food, biotechnology to pharmaceutical fields. In biotechnology, tangential flow filtration (TFF) allows obtaining the separation of different components of cells without instability phenomena. In the food industry, TFF ensures the removal of contaminants or other substances that negatively affect visual appearance, organoleptic attributes, nutritional value and/or safety of ailments. In the pharmaceutical area, purification is also an important and necessary step controlling the quality of final product. In the field of research and development of nanomedicines, several techniques are used to purify and/or to concentrate the batches for in vitro and in vivo applications. Despite many approaches that exist; current data reveal continued unsatisfactory results. Between them, TFF showed promising results, even if, currently, its use is uncommon if compared with other purification techniques usually reported in "materials and methods" sections. This review represents an overview of the different applications of TFF from protein purification to food application, with particular attention to the field of nanomedicine from polymeric to metallic nanoparticles, highlighting advantages and dis-advantages in the use of this technique. The theoretical aspect of the process has been examined.
纯化是从食品、生物技术到制药领域等不同类型方法的关键步骤。在生物技术中，切向流过滤（TFF）能够实现细胞不同成分的分离，且不会出现不稳定现象。在食品工业中，TFF可确保去除那些对食品外观、感官特性、营养价值和/或疾病安全性产生负面影响的污染物或其他物质。在制药领域，纯化也是控制最终产品质量的重要且必要步骤。在纳米药物研发领域，有多种技术用于纯化和/或浓缩批次产品以用于体外和体内应用。尽管存在多种方法，但目前的数据显示结果仍不尽人意。其中，TFF显示出了有前景的结果，即便与通常在“材料与方法”部分报道的其他纯化技术相比，目前其应用并不常见。本综述概述了TFF从蛋白质纯化到食品应用的不同用途，特别关注从聚合物纳米颗粒到金属纳米颗粒的纳米医学领域，强调了使用该技术的优缺点。还研究了该过程的理论方面。
