Biotecnology Department, Biomedics Science Institute, ICB III, University of Sao Paulo, Av. Professor Lineu Prestes, 2415, Sao Paulo, SP, CEP 05508-900, Brazil.
Food Engineering Faculty, University of Campinas, Av. Monteiro Lobato, 80, Campinas, Brazil.
Mycotoxin Res. 2018 Aug;34(3):173-178. doi: 10.1007/s12550-018-0311-8. Epub 2018 Mar 9.
Barley (Hordeum vulgare L.) is an important cereal crop for food and represents one of the main ingredients in beer production. Considering the importance of barley and its derived products, the knowledge about the mycotoxin contamination in the barley production is essential in order to assess its safety. In this study, the levels of deoxynivalenol (DON) and zearalenone (ZEN) in brewing barley were determined using a LC-MS/MS method. A survey was conducted in 2015 to estimate the mycotoxin levels in these products (n = 76) from four crop regions in Brazil. The results showed high levels of DON and ZEN in the analyzed samples, with contamination levels of 94 and 73.6%, respectively. The mean levels of DON and ZEN ranged from 1700 to 7500 μg/kg and from 300 to 630 μg/kg, respectively. Barley samples from regions 1 and 2 presented higher levels of ZEN and DON, respectively, and those from region 4 presented lower levels of both. Co-occurrence of DON and ZEN was seen in the majority of the barley grain samples, and the mycotoxin content was above the maximum levels established by the Brazilian and European regulations.
大麦（Hordeum vulgare L.）是一种重要的粮食作物，也是啤酒生产的主要原料之一。鉴于大麦及其衍生产品的重要性，了解大麦生产中的霉菌毒素污染情况对于评估其安全性至关重要。在这项研究中，使用 LC-MS/MS 方法测定了酿造用大麦中脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZEN）的含量。2015 年进行了一项调查，以评估巴西四个产区这些产品中的霉菌毒素水平（n=76）。结果表明，分析样本中 DON 和 ZEN 的含量较高，污染率分别为 94%和 73.6%。DON 和 ZEN 的平均含量范围分别为 1700 至 7500μg/kg 和 300 至 630μg/kg。来自第 1 区和第 2 区的大麦样本中 ZEN 和 DON 的含量分别较高，而来自第 4 区的大麦样本中这两种毒素的含量较低。大多数大麦籽粒样本中同时存在 DON 和 ZEN，其霉菌毒素含量超过了巴西和欧洲法规规定的最高限量。
