Department of Ecology and Ecosystem Management, Technical University of Munich, Freising, Germany.
Department of Animal Physiology & Immunology, Technical University of Munich, Freising, Germany.
PLoS One. 2018 Mar 12;13(3):e0193958. doi: 10.1371/journal.pone.0193958. eCollection 2018.
Grass pollen is the main cause of hay fever and allergic asthma in warm temperate climates during summer. The aim of this study was to determine the content of group 5 major allergens in pollen grains of agriculturally important grass species/cultivars. For each cultivar flowering dates and pollen production of cut anthers were observed in the field and in a climate chamber, respectively. An ELISA was used to quantify the group 5 allergens (Phl p5) in pollen extracts which were gained from the grass species Kentucky bluegrass, perennial rye grass, timothy, cocksfoot, annual / Italian rye grass, hybrid rye grass and festulolium. The group 5 allergen content of species varied between 0.01 ng (Kentucky bluegrass) and 0.06 ng (timothy) per pollen grain. On cultivar level the pollen allergenic content differed up to 74-times within the selected grass species. Results from this study might be helpful for the reduction of allergen exposure coming from agriculture grass production e.g. by an adapted grass selection or by the cultivation of grasses with low allergenic content in plant breeding.
草花粉是温暖温带地区夏季花粉热和过敏性哮喘的主要原因。本研究旨在确定农业重要草种/品种花粉粒中 5 组主要过敏原的含量。分别在田间和气候室中观察每个品种的开花日期和切花药的花粉产量。ELISA 用于定量花粉提取物中的 5 组过敏原（Phl p5），花粉提取物来自草地雀稗、多年生黑麦草、梯牧草、猫尾草、一年生/意大利黑麦草、杂交黑麦草和苇状羊茅。物种 5 组过敏原的含量在每粒花粉 0.01 纳克（草地雀稗）至 0.06 纳克（梯牧草）之间变化。在品种水平上，所选草种内的花粉过敏原含量差异高达 74 倍。本研究的结果可能有助于减少农业草生产中的过敏原暴露，例如通过适应性草种选择或在植物育种中培育低过敏原含量的草。
