Department of Chemistry, Stanford University, Stanford, California 94305, USA; email:
Center for Advanced Biomaterials for Healthcare, Istituto Italiano di Tecnologia, 80125 Naples, Italy; email:
Annu Rev Anal Chem (Palo Alto Calif). 2018 Jun 12;11(1):101-126. doi: 10.1146/annurev-anchem-061417-125705. Epub 2018 Mar 23.
Measurements of the intracellular state of mammalian cells often require probes or molecules to breach the tightly regulated cell membrane. Mammalian cells have been shown to grow well on vertical nanoscale structures in vitro, going out of their way to reach and tightly wrap the structures. A great deal of research has taken advantage of this interaction to bring probes close to the interface or deliver molecules with increased efficiency or ease. In turn, techniques have been developed to characterize this interface. Here, we endeavor to survey this research with an emphasis on the interface as driven by cellular mechanisms.
测量哺乳动物细胞的细胞内状态通常需要探针或分子来突破严格调节的细胞膜。已经证明，哺乳动物细胞在体外的垂直纳米结构上生长良好，它们会想方设法到达并紧紧包裹这些结构。大量的研究利用这种相互作用将探针靠近界面，或者以更高的效率或更容易地输送分子。反过来，也开发了技术来描述这种界面。在这里，我们努力调查这项研究，重点是由细胞机制驱动的界面。
