Nakamura W, Komatsu K
Hôpital Central d'Aizu, Aizuwakamatsu, Japan.
C R Seances Soc Biol Fil. 1987;181(4):458-65.
Comparison was made of the capacity of tumor cells of three different cell lines to decrease the thermotolerance in mice. Tumor cells used were that of three cell lines of mastocytoma, FMA1 and FMA3, and of Ehrlich's ascites tumor, EAT. Temperature for the assay of thermotolerance of the animal was 42.0 +/- 0.2 degrees C in the core body. Thermotolerance of animal was expressed with LD50, 42 degrees C. Tumor cells were transplanted at a dose of 10(5) cells per mouse. In the animals transplanted with tumor cells of every three cell lines, the minimal value of the LD50, 42 degrees C was obtained one or two days after the transplantation. Thermotolerance ratios (TTRs) calculated with the minimal values of LD50, 42 degrees C were 0.54, 0.19 and 0.62 for the FMA1, FMA3 and EAT cells, respectively. The thermotolerance decreasing effect of the FMA3 cells was kept unchanged even after destruction of the cells by suspending in distilled water and repeated freezing and thawing. But it disappeared partially after heating the cells for 10 min at 90 degrees C, and completely after the heating the cells for 60 min at the same temperature.
比较了三种不同细胞系的肿瘤细胞降低小鼠热耐受性的能力。所用肿瘤细胞为三种肥大细胞瘤细胞系FMA1和FMA3以及艾氏腹水瘤（EAT）的细胞。动物热耐受性测定的温度为核心体温42.0±0.2℃。动物的热耐受性用42℃时的半数致死剂量（LD50）表示。肿瘤细胞以每只小鼠10⁵个细胞的剂量进行移植。在移植了每三种细胞系肿瘤细胞的动物中，移植后1或2天获得了42℃时LD50的最小值。用42℃时LD50的最小值计算的热耐受性比率（TTRs），FMA1、FMA3和EAT细胞分别为0.54、0.19和0.62。FMA3细胞的热耐受性降低作用即使在细胞通过悬浮于蒸馏水中并反复冻融而被破坏后仍保持不变。但在90℃加热细胞10分钟后部分消失，在相同温度下加热细胞60分钟后完全消失。
