血浆长链非编码RNA HOTAIR作为胃癌的潜在生物标志物。
Plasma long non-coding RNA HOTAIR as a potential biomarker for gastric cancer.
作者信息
Elsayed Eman T, Salem Perihan E, Darwish Azaa M, Fayed Haytham M
机构信息
1 Clinical Pathology Department, Alexandria University, Egypt.
2 Internal Medicine Department, Alexandria University, Egypt.
出版信息
Int J Biol Markers. 2018 Apr 1:1724600818760244. doi: 10.1177/1724600818760244.
BACKGROUND
Long non-coding RNAs (lncRNAs) Hox transcript antisense intergenic RNA ( HOTAIR) has been suggested to be implicated in gastric cancer tumorigenesis and progression; however, little is known about the role of the plasma HOTAIR in gastric cancer diagnosis and prognosis.
OBJECTIVE
The current study was aimed at investigating the clinical relevance of plasma long non-coding HOTAIR as a non-invasive diagnostic biomarker in gastric cancer.
METHODS
Plasma HOTAIR expression was measured in 50 patients with newly diagnosed gastric cancer and 50 age- and sex-matched healthy controls using quantitative reverse transcription polymerase chain reaction.
RESULTS
Plasma level of HOTAIR was significantly higher in gastric cancer patients compared with healthy controls ( P < 0.001). By using receiver operating characteristic curve analysis, it was found that plasma HOTAIR could diagnose gastric cancer with 88% sensitivity and 84% specificity. Furthermore, increased HOTAIR expression was associated with advanced tumor stages, higher grades, and metastasis.
CONCLUSION
Plasma HOTAIR might serve as a potential non-invasive biomarker for diagnosis of gastric cancer.
背景
长链非编码RNA（lncRNA）Hox转录本反义基因间RNA（HOTAIR）被认为与胃癌的发生和发展有关；然而，血浆HOTAIR在胃癌诊断和预后中的作用知之甚少。
目的
本研究旨在探讨血浆长链非编码HOTAIR作为胃癌非侵入性诊断生物标志物的临床相关性。
方法
采用定量逆转录聚合酶链反应检测50例新诊断胃癌患者和50例年龄、性别匹配的健康对照者血浆中HOTAIR的表达。
结果
与健康对照相比，胃癌患者血浆HOTAIR水平显著升高（P < 0.001）。通过绘制受试者工作特征曲线分析发现，血浆HOTAIR诊断胃癌的灵敏度为88%，特异度为84%。此外，HOTAIR表达增加与肿瘤晚期、高分级和转移相关。
结论
血浆HOTAIR可能作为胃癌诊断的潜在非侵入性生物标志物。
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